Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,595	G→A	A229T (GCT→ACT)	melA →	alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,595	0	G	A	88.9%	17.4 / NA	9	A229T (GCT→ACT)	melA	alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/5);  minor base C (1/0);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTATGTTAACCCAATGGCGATGAATACCTGGGCGATGTATGCCCGCTATCCGCATATCAAACAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTT‑GCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGA  >  NC_000913/4342353‑4342799                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             aCTATGTTAACACAATGGCGATGAATACATGGGCGATGGATGCCCGCTATCAGCATATCAAACAGGTCGGGCTGTGCCATTCGGAGCAGGGAAGGGCGAAAGAGTTGGCGCGTGACCTCAATATAGACAAAGCTACGCTGAGTTACCGTT‑GCGCAGGTATAAACCATATGGCGTTTTACCTGGCGCTGGAGCGCAACACCGCCGACGGCAGTTATGTGAATATCTACCCAGAACTGCTGGCGCCTTATGa                                                                                                                                                                                                       >  1:219921/1‑250 (MQ=255)                                                               cAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGAAAGAGTTGGCGCGTGACCTACATATCGAACCAGCTACGCTGCGTTACCGTT‑GCGCAGGTATCAACCATATGGCGTTTTCCCTGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTATACCCAGCACTGCTGGCGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATCTTCATGGCAATACTCGCTGCCAGAATATTGTGCGCt                                                                                                                                         >  1:320227/1‑250 (MQ=255)                                                                          gtgCCATTCGGTGCAGGGAACGGCGAAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTT‑GCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCAGAACTGCTGGCGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTc                                                                                                                              >  1:274354/1‑250 (MQ=255)                                                                                 tCGGTGCAGGGAACGGCGAAAGAGTTGGCGCGTGACCTCAATATCGCCCCAGCTACGCTGCGTTACCGTT‑GCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCAGAACTGCTGGCGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGc                                                                                                                       <  1:21879/250‑1 (MQ=255)                                                                                                gcgaaaGATTTGTCGCGTGCCCTCAATTCCGACCCAGCTCCGCTCCGT‑ACCTTTTGCGCAGATATCAACCATTTGGCTTTTTACCTGTAGCTGGACCGCAAACCCGCCGACGGCAGTTATGTGAATCTCTACCCAGAACTGCTGGCGACTTATGAAGCAGGGCAGTCTCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCTTCAc                                                                                                       <  1:234810/247‑1 (MQ=255)                                                                                                                                                                           ggCGTTTTACCGGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCAGAACTGCTGGCGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAGTACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGt                              <  1:113419/250‑1 (MQ=255)                                                                                                                                                                                                       aCCGCCGACGGCAGTTATGTGAATCTCTACACAGAACTGATGGAGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTAATGGAAATACTCGCTGCAAGAATATTGTGCGCTACGACATGATCCAAACGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGGACCGATGGATGAggacacga  >  1:125194/1‑243 (MQ=255)                                                                                                                                                                                                           ccgACGGCAGTTATGTGAATCTCTACCCAGAACTGCTGGCGACTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACgg                                                                                                     <  1:184232/147‑1 (MQ=255)                                                                                                                                                                                                                                   acccaggaCTGCTGGCGACTTATGAAGCAGGGCAGGCAACGAAGCCGAATATGCATGGCAATACTCGCGGCCAGAATAGTGTGCGCTACGAAATGTTCAAAAAGCTGGGCGAGTTCGTCACGGAATCGTCAGAACATTTTGTTGATTACACACCGTGGTTTATTAAGCCAGGGCGTGAGGATTTGATTGAGCGTTATAAAGTACCACTGGATg           <  1:225369/206‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             ACTATGTTAACCCAATGGCGATGAATACCTGGGCGATGTATGCCCGCTATCCGCATATCAAACAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTT‑GCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGA  >  NC_000913/4342353‑4342799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑