Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,625	C→T	E264K (GAA→AAA)	kdpD ←	sensor histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,625	0	C	T	100.0%	29.9 / NA	13	E264K (GAA→AAA)	kdpD	sensor histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/10);  total (3/10)

GACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATC  >  NC_000913/723385‑723859                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           gACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGCCGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGgtg                                                                                                                                                                                                                                  >  1:176030/1‑251 (MQ=255)       gaATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATgg                                                                                                                                                                                                                            <  1:89744/251‑1 (MQ=255)                                                      ttCTCGTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGGTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTGTTTTTTCCGGTAAGCGGGGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCGACCGAGCCGGGACGCCAGCCGCGCCGCTGCGCGGGCCAGTTTTTTGCGGGCGGGGTTATGGCCGATGCAGAAAAGGATCGCGTCGCGCGTGTGCCACACTTTctctn                                                                                                                                                                              <  1:30834/250‑2 (MQ=255)                                                                      cAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCGGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCGTCGCCAGGAGGccccct                                                                                                                                                              <  1:88591/250‑2 (MQ=255)                                                                          aaGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGcccc                                                                                                                                                                <  1:301982/244‑1 (MQ=255)                                                                                                             gACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACgg                                                                                                                      <  1:86717/251‑1 (MQ=255)                                                                                                                                   ccGACGTTTTTTTTCCGGTAAGCGGGGCAGGGCAGGGGGTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGTGGACCAGTTTTTTTCTGCCGGTGTTATGGACGATGCATAAAAGGAACGCGTCGCCCGTGTGCCACACTTTTTCTTCGCCAGGATGccccccc                                                                                                                                                             >  1:11837/1‑188 (MQ=255)                                                                                                                                                                  gcagggTTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCGGCCGGTGTTATGGCGGATTCATAAAAGGATCGTGTCGCGCGTGTGCCATACGTTCTCTTCGCCATGATGCCCCCGCCAGGTGCGCATTTGCTCATCAACGCGATCGGCAGTTCGTCTCTGTGCCAGGTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTc                                                                 <  1:322966/251‑1 (MQ=255)                                                                                                                                                                       ggTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCNCTTNNNNNggnnnnnnnnnn                                                                                                                                                             >  2:198308/1‑144 (MQ=255)                                                                                                                                                                                   cacCGCGTGCCAGACGCTCCCCAGTCGTGACTCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAATAGGAGCGCGTCTCGCGTGTGcc                                                                                                                                                                                          <  1:91223/113‑1 (MQ=255)                                                                                                                                                                                                          gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGccn                         <  1:198308/251‑2 (MQ=255)                                                                                                                                                                                                                           gccgcTGCGCGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAg                                                                                                           <  1:113648/152‑1 (MQ=255)                                                                                                                                                                                                                                 gcgcGGACCAGTTTTTTGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCACTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGAGCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGACCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATc  <  1:109588/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           GACCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATC  >  NC_000913/723385‑723859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑