Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,307,202	G→A	Q200* (CAG→TAG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,307,202	0	G	A	100.0%	23.3 / NA	10	Q200* (CAG→TAG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

GAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAC  >  NC_000913/4306955‑4307341                                                                                                                                                                                                                                                        |                                                                                                                                            naGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCCTCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCtattg                                                                                                                                          >  1:36496/2‑251 (MQ=255)                                              aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTcc                                                                                               >  1:128667/1‑249 (MQ=255)                                                         ggTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAgc                                                                                                                       <  1:265787/214‑1 (MQ=255)                                                                                            gCATATCCATCACGCCACCGCCCAGGATCCCCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAACGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTACTTTCGGGGCTATTGTTCGTAc                                                                                                                                   >  1:106165/1‑167 (MQ=255)                                                                                            gCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgg                                               <  1:111493/251‑1 (MQ=255)                                                                                                                                  ggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTACTTTCGGGGCTATTGTTCGTACCAg                                                                                                                                >  1:107572/1‑132 (MQ=255)                                                                                                                                         aCAGATTAATGCTGGGGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCCCCCAGGATTGCTACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCTTTTCGCCTGCCACACCGTGTGCACCCGTCCAc  <  1:115194/251‑1 (MQ=255)                                                                                                                                                          gCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGGTTTCCGCATGGACGAAAAGATCGCGCAATGGTTAAATTCGGGGCTATTGTTCGTACCATCGGCTTAGCGCCATTCCAGAGCAATTgg                                                                                                    <  1:270206/136‑1 (MQ=255)                                                                                                                                                                       ccGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTggg                                                             <  1:208317/162‑1 (MQ=255)                                                                                                                                                                                                       ncGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCGATTGTTCGTACCAGCGGCTTAGCGCCATTCCAGAGCAATTGGCTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                       >  1:204455/2‑168 (MQ=255)                                                                                                                                                                                                                             nntCNNNNNATGNGTAATTTCGGGGCTATTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTCTCCAGGCACCCAGGATTGCCACTCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                       <  2:204455/144‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                            GAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCAC  >  NC_000913/4306955‑4307341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑