Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	839,883	G→A	S550F (TCT→TTT)	fiu ←	iron catecholate outer membrane transporter Fiu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	839,883	0	G	A	100.0%	29.6 / NA	12	S550F (TCT→TTT)	fiu	iron catecholate outer membrane transporter Fiu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

GTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATT  >  NC_000913/839698‑840111                                                                                                                                                                                          |                                                                                                                                                                                                                                     gTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCGCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCTGTGCGGTTGTCACTGTTTCCGCTGCCAAACTGCGCAAGTGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt                                                                                                                                                                                   <  1:175950/237‑1 (MQ=255)                 ctaGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCCACAGACGTTTATCCAGAACCTGACATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAACATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAgg                                                                                                                                                                                                    >  1:297200/3‑204 (MQ=255)                            cGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAgc                                                                                                                                          >  1:13855/1‑251 (MQ=255)                                         cTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAggg                                                                                                                                                                                                                          <  1:9312/158‑1 (MQ=255)                                         cTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAt                                                                                                                                               >  1:140284/1‑233 (MQ=255)                                                           atCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGccc                                                                                                            <  1:179896/250‑1 (MQ=255)                                                                 gCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACCTTGCCGTTTTCCGTCAGGTg                                                                                                                                                 >  1:11648/1‑207 (MQ=255)                                                                              gcggTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGGTGGCTTTTTGCGGTTTAAAATCGGTGGGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGAGTGCCCGACTTGGCGGTGTCGACggc                                                                                        <  1:243845/251‑2 (MQ=255)                                                                                                                                          gCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGACGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAGTATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGTTTTCCAGTTCATCAGCTTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATg                                             >  1:318367/1‑234 (MQ=255)                                                                                                                                                   ggTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAAACTGCGCAATGGCGAAGTTGTGGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACTGTGCCGTTTTCCGTTAGGTGATACAGCGCCCCGGTTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACTGTGGTGACTTGAGAACTTTTTGCCACACCAGGTGGTGAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGc                   <  1:129685/251‑1 (MQ=255)                                                                                                                                                                    nttGGCACTGTTACCGCTGCCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATAtt  >  1:93483/2‑251 (MQ=255)                                                                                                                                                                             gNNACCGCTNNCAAACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTg                                                                                                                                                 <  2:11648/99‑1 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                                     GTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCATCAGATTGCCCGACTTGGCGGTGTCGACGGTGGTGACCGGAGAACCTTTTGCCACACCAGTTGGGCAGGTGATGGCACCGCGTCCGCTGCCGCCGCAGGCGGTGGCACTGTCATATT  >  NC_000913/839698‑840111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑