Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,473,353	C→T	E54E (GAG→GAA)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,473,353	0	C	T	100.0%	36.0 / NA	14	E54E (GAG→GAA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

ACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGCTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTGCGCACTGATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTT  >  NC_000913/3473159‑3473548                                                                                                                                                                                                   |                                                                                                                                                                                                    acacCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGGGAAGTCAACGTGCCCCGGGGTGGCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAAGGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGaaat                                                                                                                                                                 <  1:312138/231‑3 (MQ=255)                                  ccATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGTAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCan                                                                                                                                                             <  1:39176/202‑2 (MQ=255)                                        agaACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGCTGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGtagtagagatctg                                                                                                      >  1:89374/1‑244 (MQ=255)                                                             aCTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGGTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAAGGCAGTAGTCGCAACGGAAGTGATGGTAATACCACGTTCCGGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGGCATGAACTTCACCGATTGTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTgcgc                                                                                    <  1:321581/248‑1 (MQ=255)                                                              cTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATCATGTTGATGCGATGCGGCTCATACTGCTTAGACATACCAGACCCGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCCTACAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATAAGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTGCGCACTg                                                                                >  1:216542/1‑251 (MQ=255)                                                                                     cccGGGGTGTCGGTGATGTTGATGCGATGAGGCTCATCATGCTTAGCCATACCAGACCCGCCTGCAGTCGACGTAGCGGAAGTGATGGCACTACCACGTTACTGATACTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCCACGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTCGTAGTGGTTTTCCCGGCCTCGATGTGCGCAc                                                                                  >  1:310510/1‑226 (MQ=255)                                                                                                        tGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACAAGAATGCAGTAGTCGCAGCGGAAGCGATGGTAATACCACGTTACTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGCACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGCTGTGCGCACTGATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGcc                                      >  1:132728/1‑251 (MQ=255)                                                                                                                       cATACTGCTTAGCCATACCAGACCAGAATGCAGGAGTCGCAGCGGAAGTGAGGTTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAAc                                                                                                                                                                  <  1:229486/112‑1 (MQ=255)                                                                                                                       cATACTGCNTNNNCNNACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACNACGTTCCTGCTCCTGNTCNATCCNGTCNATGGTTGCAGCGCCGTCATGAAc                                                                                                                                                                  >  2:229486/1‑112 (MQ=255)                                                                                                                                     aTACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGGTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACTCCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTGCGCATTGATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGt         <  1:331194/251‑1 (MQ=255)                                                                                                                                             ccagaATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTGCGCACTGATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAAttt  >  1:242442/1‑250 (MQ=255)                                                                                                                                               agaATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGcc                                                                                                                                                                           <  1:257748/79‑1 (MQ=255)                                                                                                                                                                gCGGAAGTGATGGTAATGCCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTcn                                                                                                                                                                       <  1:202986/66‑2 (MQ=255)                                                                                                                                                                gCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTTGCAGCGCCGTCa                                                                                                                                                                       >  2:202986/1‑66 (MQ=255)                                                                                                                                                                      gTGATGGTAATACCACGTTCCTGCTCCTGTTCCATCCAGTCCATGGTGGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTgc                                                                                      <  1:131896/141‑1 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                    ACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCTGCTCCATCCAGTCCATGGTTGCAGCGCCGTCATGAACTTCACCGATTTTATGGTTTACACCGGTGTAGAACAGAATACGTTCGGTAGTAGTGGTTTTACCGGCGTCGATGTGCGCACTGATACCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTT  >  NC_000913/3473159‑3473548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑