Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,387,757	C→T	T538T (ACG→ACA)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,387,757	0	C	T	100.0%	28.9 / NA	13	T538T (ACG→ACA)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

TCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGG  >  NC_000913/4387564‑4387998                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  ccatcaGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTgcgc                                                                                                                                                                                          <  1:301266/250‑1 (MQ=255)             agCGCATCCCACGGGAAACACGTTAGCGCGGCCAGCCAACATGAGCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGAAAATCATCACTACCAACAGCAGCGGCACGGTGGCCGTTACACCCTCGCCAATCGCGACCGCCAGCGGCTAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGCAAAGAGTgcgc                                                                                                                                                                                          >  1:183197/1‑239 (MQ=255)                                 ctttCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCAGTTCGGGCGGGCCAAGGTGGCGCAAATCATCACTACACACCGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCACGCCGTAACACAGTGTCATCCACAGCACCGGTAACGGTGACGCGCCGAGAATCGACCAGAAAAGAGAGCGCAAAGTCAGCCAGAAGTGATCCTg                                                                                                                                                                   >  1:301035/3‑242 (MQ=255)                                                ccaAAATGCGCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTcgccgc                                                                                                                                           >  1:102407/1‑251 (MQ=255)                                                         gCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCCGCGCCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGACTCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCAGAACGTTc                                                                                                                                  >  1:290882/1‑251 (MQ=255)                                                                                               aaTCATCACTCCCCACAGCAGCGCCACGGGGGCCGTTACCCCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCGCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACTCGACGAGAATCGACCAGAAAAGAGGGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGc                                                                                            <  1:286875/251‑1 (MQ=255)                                                                                                   atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCCACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGCAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGACAACGGGTGAAATAGCGGCGCGAGTAAATAATGCAAcc                                                                                        >  1:284993/1‑251 (MQ=255)                                                                                                                   gcGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCCCAGCACCGGTACCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGACCGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAAcgcg                                                                        >  1:206933/1‑251 (MQ=255)                                                                                                                             ggcctttaccccacagCCAACCGCGACCGCCAGCGGATAAGGCCACGCCACGCACAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGTTGACGCTACGAGAACCGACCAGAAAAGAGTGCTCAACGTCAGCCAGAAGTGATCCTGATTCACTGTGCCAACTTTCGACGCCGAACGTTCAAGAAAACGGGTGAAATATCGGCGCGAGTAAATTCTGCAACCTACCAGAATCAACGCGCTAAACAGc                                                               <  1:54646/235‑1 (MQ=255)                                                                                                                                       ccATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAg                                                                                                                                                                                  <  1:10554/125‑1 (MQ=255)                                                                                                                                            gCCAATCGCGACAGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCAACAGCACCGGTAACGGTGCCGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGCGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGTAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCtt                                               >  1:214242/1‑251 (MQ=255)                                                                                                                                                                    aGGCCACGCCTCGCGCAAGCCGTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCTCAACGTCAGCCAGAAGTGATCCTGAGCCACTTTGCCAACTGTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCaa                                                                                          <  1:150442/184‑1 (MQ=255)                                                                                                                                                                                         gTAACCCAGTGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCTGCGCGAGTAAATACTGCAGCCGGCCAGAATCAACGCGCCAAACAGCTGCAAAATTGTCGCTTTGCTGGTCAGCAGCATCACACTGGCTTTGCCCAACTGACTGAAgn  <  1:28335/251‑2 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  TCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGG  >  NC_000913/4387564‑4387998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑