Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,894	G→A	intergenic (‑278/‑338)	yaiS ← / → tauA	putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,894	0	G	A	100.0%	37.9 / NA	13	intergenic (‑278/‑338)	yaiS/tauA	putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

CTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCAC  >  NC_000913/384680‑385054                                                                                                                                                                                                                       |                                                                                                                                                                 ctGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATg                                                                                                                                  <  1:128743/247‑1 (MQ=255)                                                 agAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTta                                                                                                                                                   <  1:12456/181‑1 (MQ=255)                                                      aGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTTGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGtt                                                                         <  1:92037/251‑1 (MQ=255)                                                                                               tCTATGCAATTCATATGTTAAGTGTTNGTATGTNNGGNATGTATAGTTATTTTGTTTNATNNNTNNNANTTAGANGTNNTTTAGTTTGNCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACANTATGTGAATNNAATATGCGAGTGATTGAGAATNATANNGNNANATANAcn                                                                                          >  2:72708/1‑192 (MQ=255)                                                                                               tCTATGCAATGCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGGTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAAGTAAGTGCATTTAAAAAATATGTTATGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAAGATAATAGTTTTATTATATGTATTTATATGGATAg                                                <  1:72708/235‑1 (MQ=255)                                                                                                                            aTGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCAtn    <  1:192796/250‑2 (MQ=255)                                                                                                                              gTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCAc  <  1:12454/250‑1 (MQ=255)                                                                                                                                                                             aTTTAGTTTGTCAATTAATTGCATTTAAAAAAGATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTTATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGttt                                                                        <  1:303272/133‑1 (MQ=255)                                                                                                                                                                                  gTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATAGGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATc    <  1:183721/196‑1 (MQ=255)                                                                                                                                                                                  gTTTGNCANNTANNTGCNTTGAAAAAATATGTTCTGTAAACAAGCATTGTTtanan                                                                                                                                                >  2:183721/1‑55 (MQ=255)                                                                                                                                                                                       tCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGtnttgnnnnngann                                                 >  2:192558/1‑144 (MQ=255)                                                                                                                                                                                       tCAATTAATTGCATTTAAAAAATATGTTCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATCTTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTan         <  1:192558/186‑2 (MQ=255)                                                                                                                                                                                                                  tCTGTAAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTa                       >  1:15607/1‑145 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                 CTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCAC  >  NC_000913/384680‑385054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑