Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,264	C→T	E115K (GAA→AAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,264	0	C	T	100.0%	37.0 / NA	13	E115K (GAA→AAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

AGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCA  >  NC_000913/2003031‑2003457                                                                                                                                                                                                                                          |                                                                                                                                                                                                  aGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTCTCGTTATTTTTAAAGCTAAAACCATCAAGGCCAAGAGTTTTAGCCTCAATCTGATTCCGATCGATAGTGATAGTCTGGTTCTCATTTGCGCAACCCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGCTTTAATTTTGTCCTGGATAGAAGAc                                                                                                                                                                                   >  1:317158/1‑250 (MQ=255)     ccAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATAAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTACTTTTGTCCTGGCTAGAAGAc                                                                                                                                                                                   >  1:219524/1‑246 (MQ=255)                                          cGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTAAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTc                                                                                                                                         >  1:41310/1‑251 (MQ=255)                                           gTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGATATCATTTGCGCCAACCTGGACTTTCATGGAGCCATTTTTTGCCAGCACGTTCCCGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTc                                                                                                                                         >  1:156743/1‑250 (MQ=255)                                           gTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTACAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCCCGTTCACGCCGTTGAAATGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTc                                                                                                                                         >  1:176914/1‑250 (MQ=255)                                                           aaaCCATCAAGGCCAAGAGTTTTAGCNTCAATCNNCTNCAGATCGATAGTGATAGTCNGGNNNTNNNTNGCGCCNACNNGGCTTTTCANGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATANGCGGTCCATNNCATCCAGACGGGATTTAATTTNGTCNNGNNTNGAANAcn                                                                                                                                                                                  >  2:70879/1‑192 (MQ=255)                                                                       ccAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgtggttgtt                                                                                                            <  1:70879/251‑1 (MQ=255)                                                                                    cATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATGTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGtt                                                                                                                                     <  1:181506/213‑1 (MQ=255)                                                                                                                            aTTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTAGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCg                                                                                                     <  1:324351/205‑1 (MQ=255)                                                                                                                                  nccAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGCAAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTAc                                                 >  1:65215/2‑251 (MQ=255)                                                                                                                                                                  ccAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGCCTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTag                   >  1:314356/1‑249 (MQ=255)                                                                                                                                                                    aGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGGTGGCGTTACGGGCCGCCt                                        <  1:116244/226‑1 (MQ=255)                                                                                                                                                                             ccGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAAGGTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTTGCCTGTTCCGTCAGTTCACGCACACGCTGTAAGTTGTTGGTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGtt      <  1:219460/250‑1 (MQ=255)                                                                                                                                                                                  gTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCa  >  1:113227/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                  AGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCA  >  NC_000913/2003031‑2003457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑