Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,743	C→T	D78N (GAT→AAT)	yeaC ←	DUF1315 domain‑containing protein YeaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,743	0	C	T	100.0%	29.0 / NA	12	D78N (GAT→AAT)	yeaC	DUF1315 domain‑containing protein YeaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

GAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCC  >  NC_000913/1861523‑1861903                                                                                                                                                                                                                             |                                                                                                                                                                 gagGGATTACCGGCCAAAGAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAAGTGAGATGAGTCGTTTGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGTACCACTTACTTAAACATTGCGATAGGCGTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGan                                                                                                                                    <  1:36905/251‑2 (MQ=255)      aTTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATtttctt                                                                                                                                                             <  1:266982/221‑1 (MQ=255)                                     tCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCAGTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATATGCTTTGCTGAGATACCAAAATTTTCGTTTAACTGCTGGTTGCTCTTCATGACCATCTGACCAATTGTGTCAATTGTCATATGCtgtg                                                                                                 <  1:207487/250‑1 (MQ=255)                                         gagaAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCGTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCg                                                                                            <  1:137340/251‑1 (MQ=255)                                                       gtgtGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGc                                                                              <  1:20956/251‑1 (MQ=255)                                                                ggACGGCAAGAGATCGGACTAGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCtn                                                                                                                              <  1:37047/194‑2 (MQ=255)                                                                              cGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTgn                                                        <  1:204924/250‑2 (MQ=255)                                                                                      gACTTAGCCCGGTCATGGTGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCtt                                                                                                                                          >  1:87998/1‑160 (MQ=255)                                                                                                                                                   cTTCATAGCCGAGGCGAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGGTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGcc                                                                         <  1:329491/164‑1 (MQ=255)                                                                                                                                                   cTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATCCTATGGCGGGCTTTCCACAGCaacc                                                                >  1:319360/1‑170 (MQ=255)                                                                                                                                                            cGAGGCAAAAAGGGGCTGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGAtatta                                                                                      >  1:114055/1‑142 (MQ=255)                                                                                                                                                                                             cATGGCAATAGGCTTTGCTGAGATACCAAAATTGTCTTTTAACTGCTGTTTGCTCTTCATGACCAGCTGACCATTTGGGTCAATTGTCATATGCTAGGCTTCGATATTATGGCGGGCGTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTcccc  <  1:212115/193‑1 (MQ=255)                                                                                                                                                                                                 ncAATAGGCTTTGCTGAGATACCAAAATTTTCTTTTacc                                                                                                                                                        >  1:35034/2‑37 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                 GAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCC  >  NC_000913/1861523‑1861903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑