Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,332	C→T	E103K (GAA→AAA)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,332	0	C	T	100.0%	53.3 / NA	19	E103K (GAA→AAA)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

CAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTCCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAG  >  NC_000913/3513092‑3513537                                                                                                                                                                                                                                                 |                                                                                                                                                                                                              cAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTACACTGCGTCATGCGACTCAGTTTGCCGAAGt                                                                                                                                                                                                       >  1:22548/1‑249 (MQ=255)          gAATAAACCGCTCCGGAAACATAAAGACCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCATTTAGAGGTGCTGTTTCTGGTCTTCACCCACCGGTTCCAGATTAGTGTGATACAGCAGGATGTCTGCTGCTATCAGCACCTGATAGTTAAACAGACCAGCGTTGATGGTCTCGGCACAACGCCCAGATCTATCTTTAATCTGCGTCATGCGTCTCAGTTTGCCGACGTAGGTATAGCAg                                                                                                                                                                                            <  1:230880/251‑1 (MQ=255)                                                                             ncTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCACAAACAGATTAGTTTGATAAACCAGGATGTCCGCTGCCATCAGCACCAGAGACTCAACCAGCCCAGCGTAGATGTTCTCGGCATCACGCGCCGATTTATCTTTAAACTGCGTCATGTGACTCAGTATGCCGACATAGGTATAGCAGTTCAGTGCCCAGCCTAACAGTGCATGTTTCGGCACATGGGACTGAACAAAAATGGTGCTCTtctc                                                                                                                           >  1:63418/2‑249 (MQ=255)                                                                                      gAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCAGCGGATAGTCACACAGAACAGCGTTGATGTTCTCGGCATCACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATAc                                                                                                                >  1:183757/1‑251 (MQ=255)                                                                                       aGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTGCTCGGCATAACGCGCAGATTTAGCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGTATCTATAcc                                                                                                               <  1:264024/251‑1 (MQ=255)                                                                                                       cTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACaa                                                                                                >  1:18065/1‑250 (MQ=255)                                                                                                          cacccaccGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCCCCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGAGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGACCAGCCTAACTGTGCATGTTTCGGCACGTGGGCATGAACAAAAATGGTGCTTTTCTCAGGATAGATACCACAAGCCAGATACAAGGcc                                                                                            >  1:315369/1‑251 (MQ=255)                                                                                                                 cGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAATGCGCAGATTTATCGTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCgg                                                                                                                                                            <  1:212000/180‑1 (MQ=255)                                                                                                                         gATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCTCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGcc                                                                                                                                                                                                            <  1:165266/124‑1 (MQ=255)                                                                                                                              gTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCCGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCTTATCCAGCGTCGCttt                                                                        >  1:189234/1‑251 (MQ=255)                                                                                                                                 tGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTGCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTAc                                                                      <  1:164580/250‑1 (MQ=255)                                                                                                                                                      nccATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGCTTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTgg                                                  >  1:261205/2‑249 (MQ=255)                                                                                                                                                        cATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCCGCTTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGgcgc                                               >  1:119196/1‑250 (MQ=255)                                                                                                                                                                ccGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTCTCTTTAAAATGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTCACTGTGCATGTTTCGGCACGTgg                                                                                                                                                     >  1:172952/1‑140 (MQ=255)                                                                                                                                                                                                  ggCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGTATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGGGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGt    <  1:153987/251‑1 (MQ=255)                                                                                                                                                                                                                         tAAACTGCGTCATGCGCCTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCGAACTGTGCATTTTTCGGCACGTCGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATAcc                                                                                                               <  1:43474/121‑1 (MQ=255)                                                                                                                                                                                                                                    aTGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTan  <  1:61471/219‑2 (MQ=255)                                                                                                                                                                                                                                       cGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCGCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCtg                                                            <  1:183143/158‑1 (MQ=255)                                                                                                                                                                                                                                        gACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTCTGCATGTTTCGGCACGTGGCACTGAAAAAAAATGGTACTTTTATCAGGCTCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGc                                                                           >  1:321497/1‑142 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                              CAGATTTCGGAATAAACGGCTCCGGCACCTTAAAGATCTCGCCATACAGCGCGTTGAAACGCTGGGCAATATCGCGGCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTCCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAG  >  NC_000913/3513092‑3513537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑