Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	678,229	C→T	A272V (GCA→GTA)	djlB →	J domain‑containing protein DjlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	678,229	0	C	T	100.0%	17.0 / NA	9	A272V (GCA→GTA)	djlB	J domain‑containing protein DjlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/7);  total (2/7)

ATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGCAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTC  >  NC_000913/678001‑678451                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               aTCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGCTGCAAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGGGCCTTATTTTGAATACGCCCACCGCGCCTATTTTGGGGAAGGCGAAAGCCTGTTGGTAGAACTTtgtg                                                                                                                                                                                                                     >  1:135165/1‑240 (MQ=255)                                               tATCCCCGATGATGCAAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTNc                                                                                                                                                             <  1:253450/250‑1 (MQ=255)                                                   cccGATGATGCAAAACTGCAAAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAAACTGATAATGAAAAAGCGCCTTATTATGAATACGCGCAACACCTCTATTGTGGAGAAGGCGAAAGCATGTTGGTAGAACTTTTTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGGTGCTTTTATTTTGCAATGGTGCCGTc                                                                                                                                                         >  1:268285/1‑250 (MQ=255)                                                                            aTTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTAccc                                                                                                                                   <  1:236092/247‑1 (MQ=255)                                                                                                                 ccccGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGGCAGCATCGGGTCGATTATTACCCAGTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACg                                                                                          <  1:171459/251‑1 (MQ=255)                                                                                                                              gATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGGTGGTAGAACTTGGTGACTACTGGCGCGAGTATCCCTCCAGCCAGGCGGATGCTTTAATGGTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGcgcg                                                                                                        <  1:190823/224‑1 (MQ=255)                                                                                                                                               gCGGGAAACTGAACCTGATAATGAAGAGGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACt                                                                                                                                                                                                                          <  1:146961/93‑1 (MQ=255)                                                                                                                                                                      aaGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTTCTGGTGATGATGATTGAAGCGCGTGATCTGTTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGtttt                                     <  1:127495/251‑1 (MQ=255)                                                                                                                                                                                                          aTTGTGGAGAAGGCGAAAGCCTGTTGGTAGAACTTTGTGACTACTGGCGCGATTATCCCTCCACCCAGGCGGATGCTTTAATGGTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTTCTGGTGATGATGGTTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCGACTTTATTTCCCCGGGGACTGCGTCCGTACGCGTGTTCATTTATACGAAATACTCAGCGATGAAAAActctc  <  1:300444/250‑1 (MQ=255)                                                                                                                                                                                                                               tGTTGNNAGNNNTTNNTGACTACTGGCGCGAGTATCCCTNCACCCAGGCGGATGCTTTAATGTTGCAATNGNGNCGTCNNCATNGGGTCGATTANTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATNANGGAAAACCGCTACTTTATGTCCCCGGCGACANCGCCCGTACGCGTTTTCATTTATANGAAANNccnan                 >  2:238228/1‑210 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               ATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAAGCCTGTTGGCAGAACTTTGTGACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTC  >  NC_000913/678001‑678451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑