Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,854	G→A	V68M (GTG→ATG)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,854	0	G	A	100.0%	15.7 / NA	7	V68M (GTG→ATG)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATGACAGTTCTGGAGTGCAGGCGAAAAAACACTACGGCTCCATGTACGAACCCATCCTGATGATGGTGAAAGACGCAAAGAACTACACATTCAACGGTGATGCTATTCTGG  >  NC_000913/3411620‑3412097                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    nccTTTTTTATAGGTGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGACATGATTGCAGAGTGcc                                                                                                                                                                                                                                      >  1:80994/2‑250 (MQ=255)                                                                                                   gtttGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAATTCTGTTTATCGACTGTCTGTTTGAAATGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa                                                                                                                                                                                              <  1:1397/190‑1 (MQ=255)                                                                                                                 aaaaGAACCCCGCCGAAAGTGTCTATCTGATCTGTGCCGACCCACCATATAACATCGGTAAAAATTTTGAGGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAATGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCCCGCTTTTTACCATCAAAAGTCGCATCGTCTgn                                                                                                                     <  1:205137/251‑2 (MQ=255)                                                                                                                        ccccGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGTTAAAAATTTTGATGGTCTGAGCGAAGCCTGGAAAGAAGATCTGTTTATCTACTGGCTGTTTGAAATGATTGCAGAGGGCCACCGCTTTCTGAAAAATCAGTGCAGCATGTACATCTTTAACAGGACGGAAAACATGCCCTTGATCGATCGCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATg                                                                                                              <  1:223514/251‑1 (MQ=255)                                                                                                                                                                  taACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAATGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATGACAGTTCTGGAGTGCAGGCGAAAAAACACTACGGCTCCATGt                                                                    >  1:282217/1‑251 (MQ=255)                                                                                                                                                                          gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAATGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATGACAGTTCTGGAGTGCAGGCGAAAAAACACTACGGCTCCATGTACGAAccc                                                            >  1:163399/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTTTGAAATGATTGCAGAGTGCCACCGCGTTCTGACAACGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATGACAGTTCTGGAGTGCAGGCGAAAAAACACTACGGCTCCATGTACGAACCCATCCTGATGATGGTGAAAGACGCAAAGAACTACACATTCAACGGAGATGCTATTCTgg  >  1:87119/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCATCAAAAGTCGCATCGTCTGGTCATATGACAGTTCTGGAGTGCAGGCGAAAAAACACTACGGCTCCATGTACGAACCCATCCTGATGATGGTGAAAGACGCAAAGAACTACACATTCAACGGTGATGCTATTCTGG  >  NC_000913/3411620‑3412097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑