Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,417	G→A	L268L (CTG→TTG)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,417	0	G	A	92.3%	29.4 / ‑6.6	13	L268L (CTG→TTG)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/3);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGG  >  NC_000913/4316216‑4316659                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   gATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACgtggtg                                                                                                                                                                                                   <  1:265710/251‑1 (MQ=255)                               ncAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCTGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTgg                                                                                                                                                                     >  1:36553/2‑251 (MQ=255)                                           ggTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGAGGGGTAGTAGTTGGAAGCGAGGATATCCAGCAGGCCAAGCCGCGCCAGTTCACTGGCCGCCACCATGCCGGAGTGCGAGACGCCGCACACAATATTCGGCGCGCCCATCAAAACGCTCATGCCATGCTTGCGCGAGGCTTACGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCCCGc                                                                                                                                                                                >  1:216020/1‑228 (MQ=255)                                                     gTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAAcg                                                                                                                                                      <  1:105994/244‑1 (MQ=255)                                                            ncGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGt                                                                                                                                                                                                                                               >  1:60996/2‑148 (MQ=255)                                                                                                tagtagTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTcgcgcgcg                                                                                                    >  1:182699/1‑251 (MQ=255)                                                                                                                                              cACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgcg                                                      >  1:112522/1‑251 (MQ=255)                                                                                                                                                              gCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGCGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGgacggc                                                                                        >  1:70471/1‑201 (MQ=255)                                                                                                                                                                      ncGNGCCNCNNCNNACANTATTCGGCGCGCCCATCAGCANNTTCATGCCANGCTTGCGCGAGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGNTGCCAAGCTNNGGNGATTCNGNNNCNNNGGNGTGGGTGGCGTCATCGTGGNTGNNAAGCGCNATTTTGCGCGCGCGGCGCAGgacggc                                                                                        <  2:70471/192‑1 (MQ=255)                                                                                                                                                                                                 ngcCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGATGGTGAGATTCAGAAACGTGGGCGTGGGCGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTg      >  1:62900/2‑248 (MQ=255)                                                                                                                                                                                                  gcCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAAtt                                                                                                               >  1:3876/1‑142 (MQ=255)                                                                                                                                                                                                   cccATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGCGAAATTCGGCGCTCACGCTGCCACGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGGCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAggg  >  1:148198/1‑250 (MQ=255)                                                                                                                                                                                                      aTCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGc                                       <  1:251954/210‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   GATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGG  >  NC_000913/4316216‑4316659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑