Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,085	C→T	G130S (GGC→AGC)	mipA ←	MltA‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,085	0	C	T	100.0%	23.4 / NA	11	G130S (GGC→AGC)	mipA	MltA‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

GTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGT  >  NC_000913/1865894‑1866326                                                                                                                                                                                                |                                                                                                                                                                                                                                                  gTCGTTCGGGTTATAGCCACGCAGCCCTCTGCGAGCGGACTCCTTGCGCTATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGGCACGGTCAGGCCACCGTTGGTGTAACGAGACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCATGGTATCGCTAGACAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAgt                                                                                                                                                                                          <  1:246696/249‑2 (MQ=255)       cGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATg                                                                                                                                                                                   <  1:111701/250‑1 (MQ=255)                                 gAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGGCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCGGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGGGAGCATAAGACAGACCAGCCATCAGGGTGCTCTTACGGTCATCCAGGTGACGc                                                                                                                                                        <  1:168342/251‑1 (MQ=255)                                                                                tCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATACCCCAGACGATTCCGTTGCTGTTATCCAGTGTATCGCTAGCCAGTGTGGTACGCAGGTAACCGTACTTGGTAAAGGGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCAACCAGGTTGCGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGAcc                                                                                                          <  1:238095/250‑1 (MQ=255)                                                                                  gCTGTTCCACTGCACACCAATACCCGGAGTCCCGGTCAGGCCACCGTTGGTGTAACGCTCCAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCTAGCCCGGGCGGTACGCAGGTCACCGTACTGGGTAACGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATc                                                                                                                                                                  >  1:293420/1‑192 (MQ=255)                                                                                                                cACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAagacagac                                                                                                                                                                                              >  1:6615/1‑134 (MQ=255)                                                                                                                         gCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCATCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATc                                                                                 <  1:245385/234‑1 (MQ=255)                                                                                                                                              cAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGc                                           <  1:284586/251‑1 (MQ=255)                                                                                                                                                                      gCCGTTGCTGTTATCCAGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGCGCATAAGACAGCACAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCcaca                                                                >  1:244922/1‑206 (MQ=255)                                                                                                                                                                                      aGGGTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCgg   >  1:91842/1‑251 (MQ=255)                                                                                                                                                                                       gggTATCGCTAGCCAGGGTGGTACGCAGGTAACCGTACTGGGGAAAGTGCGCATAAGACAGACAAGCAATCATGGTGATCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGAGGCGAACAGTAAGCGGTAATTGAACGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAAACCGACGTTATCGCCTTCATAGTTGATTACCGGt  >  1:316538/1‑251 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                                  GTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGT  >  NC_000913/1865894‑1866326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑