Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	467,730	G→A	D107N (GAC→AAC)	cof →	HMP‑PP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	467,730	0	G	A	100.0%	35.6 / NA	13	D107N (GAC→AAC)	cof	HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/10);  total (3/10)

CGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA  >  NC_000913/467513‑467953                                                                                                                                                                                                                          |                                                                                                                                                                                                                                ngACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTg                                                                                                                                                                                                >  1:194141/2‑251 (MQ=255)           cTCACTTTTGCCACGGGGCGTCATGTGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCgn                                                                                                                                                                                                              <  1:61056/227‑2 (MQ=255)                                                                gcTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGTTTTTCTTTATCAGATAATCGATGTCAAAAAAATGc                                                                                                                                 <  1:259195/251‑1 (MQ=255)                                                                              cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAATGTGAACTTTTACATCGTGATGATTTACCTGCGGAGGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTGGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATgn                                                                                                                                 <  1:93468/237‑2 (MQ=255)                                                                                                aCGGAACGCGCGTGCATTCTCTGGTAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGTAGCTGGTGCTGTATCAGCAATGGGATTCCCGAGCCAGCATGCATATCTGCAATAACGACGGTTGGTTTACCGGGAAAGAGATCTCTGCGTTGTTGCAGTCATTTGTCTATAACGGTTTTCTTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgt                                                                                                 <  1:237583/251‑1 (MQ=255)                                                                                                          cgTGCATTCTCTGGAAGGTGAACTTTTAAATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTgg                                                                                               >  1:272584/1‑243 (MQ=255)                                                                                                                                                                  cgGATCTGGTGCTGTATCAGCAATGTGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCg                                                                                                                                                                                              <  1:50294/92‑1 (MQ=255)                                                                                                                                                                            gCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGTCGATCGTGCACATTTGTGtttg                      <  1:90693/250‑2 (MQ=255)                                                                                                                                                                                tagcatccatgtgagaCCCGGGCCAGCATTCATATCGTCAATAACGACGGTTGGCTTACCGGGAAAGATATCACTGTGTTGTTTCAGGCCTTTGTCTATAGCGGCTTTCGTTAGCAGAGAATCGATGTCAAAAAAATGTCACTCAGCAGCGTCACCAAGATCTGCGTCTGTGGCGATCACGAAGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCg                  <  1:142486/235‑1 (MQ=255)                                                                                                                                                                                      nnnnnnnnnnccnNNNNCAGNATGCATATCTTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGa                                                                                                            <  2:194141/144‑1 (MQ=255)                                                                                                                                                                                                              catATCTTCAATAACGACTGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGTCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCGGCTTCTGTTGCGATCACGACGATCTTACATGCGGGCAGATCCAGCTAGACGAATCATTAGGGGGTCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGa  <  1:290695/236‑1 (MQ=255)                                                                                                                                                                                                              catATCTTCAATAACGACGGATGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTcc                   >  1:119113/1‑219 (MQ=255)                                                                                                                                                                                                              catATCGTCAATAACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGc                                                                                                                                                                                   <  1:102476/59‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                CGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA  >  NC_000913/467513‑467953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑