Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,808	C→T	I34I (ATC→ATT)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,808	0	C	T	100.0%	31.4 / NA	13	I34I (ATC→ATT)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

AAATTCAATTGCTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACA  >  NC_000913/4437594‑4438048                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 naaTTCAATTGCTAATAAGTTACATTTTAATCATGAGCGTTTTTTGATAGTTTACTTATATAGTGAGATATTTAATGGCGAAATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACAAAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAAAAAAATTAATCATGACTTTAAGACAAAAGATTGGATTTCGAAt                                                                                                                                                                                                              >  1:33134/2‑251 (MQ=255)                                                                 gagaTATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCCGACAAAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTATTCGTATAAAAAAACACATTCAAACAATTTTTTTAACAACTTTCATCATGCATTTAAGAAACAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGCGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGtt                                                                                                                                              >  1:326842/1‑251 (MQ=255)                                                                                                                  atGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTAAACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAgn                                                                                              <  1:196137/250‑2 (MQ=255)                                                                                                                                                   ggTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAg                                                                                                                                                                                       <  1:175359/128‑1 (MQ=255)                                                                                                                                                      gCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCg                                                          >  1:90182/1‑250 (MQ=255)                                                                                                                                                        ggTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAACGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAat                                                       >  1:166136/1‑251 (MQ=255)                                                                                                                                                              gTTATTTGTAAAGTCTTCGTATATAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCt                                                                                          <  1:12397/210‑1 (MQ=255)                                                                                                                                                              gTTATTTGTAAAGTCTTCGTATATAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTNNGAATTa                                                                                                                                                                                                            >  2:12397/1‑96 (MQ=255)                                                                                                                                                                            cTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAggg                                                                                                                                                                                     <  1:20767/105‑1 (MQ=255)                                                                                                                                                                                gTATACAAAAACACATTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGtn                               <  1:84197/251‑2 (MQ=255)                                                                                                                                                                                             caTTCAAACAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATACCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATCAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATCTGAATACg                   >  1:310997/1‑250 (MQ=255)                                                                                                                                                                                                  aaaCAATTTTTTTAACAACATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGACTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAg                                                                              >  1:303953/1‑186 (MQ=255)                                                                                                                                                                                                              naacaacATTCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGGTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACa  >  1:203722/2‑250 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 AAATTCAATTGCTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACA  >  NC_000913/4437594‑4438048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑