Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,201	G→A	G168D (GGC→GAC)	ynfM →	putative transporter YnfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,201	0	G	A	100.0%	24.3 / NA	9	G168D (GGC→GAC)	ynfM	putative transporter YnfM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

TGGTCGCAAACCAGTGATGGTCACGGCGCTACTGTTGGCCTCCATTTGTACGTTACTTTCGACAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGACTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGGCAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTCAACTGGCGAATTGCTCTGGCGGCAATCGGTTGTTTCGCGCTGG  >  NC_000913/1669992‑1670302                                                                                                                                                                                                                  |                                                                                                      tGGTCGCAAACCAGTGATGGTCACGGCGCTACTGTTGGCCTCCATTTGTACGTTACTTTCGACAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGTCTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGTCTTTTCAATGGTGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGATGCTTAATTAGCGgt                                                               <  1:47961/250‑1 (MQ=255)                                  gTTGGCCTCCATTTGTACGTTACTTTCGACAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGACTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTCAACTGGCGAATTGc                               <  1:71982/249‑1 (MQ=255)                                                             naCAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGACTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTCAACTGGCGAATTGCTCTGGCGGCAATCGGTTGTTTCGCGCTgg  >  1:192776/2‑251 (MQ=255)                                                                               gCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGACTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTCAACTGGCGAAt                                  >  1:211186/1‑201 (MQ=255)                                                                                                                                                    ttATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTc                                             <  1:15552/121‑1 (MQ=255)                                                                                                                                                                              gTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACg                                                      <  1:25096/86‑1 (MQ=255)                                                                                                                                                                              gTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACg                                                      >  2:25096/1‑86 (MQ=255)                                                                                                                                                                                        cTTTTCAATGGGGTTGTATCTCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCAc                                                       >  1:7369/1‑75 (MQ=255)                                                                                                                                                                                        cTTTTCAATGGGGTTGTATATCAGCGACAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCAc                                                       <  2:7369/75‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                      TGGTCGCAAACCAGTGATGGTCACGGCGCTACTGTTGGCCTCCATTTGTACGTTACTTTCGACAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGATTGGGCTTTCGTTAAGTGGCGTGGCAGCTGTTGGCATGACTTATCTTAGCGAGGAAATCCATCCCAGTTTCGTGGCCTTTTCAATGGGGTTGTATATCAGCGGCAACTCAATTGGCGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTTCAACTGGCGAATTGCTCTGGCGGCAATCGGTTGTTTCGCGCTGG  >  NC_000913/1669992‑1670302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑