Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,309,387	G→A	T28I (ACC→ATC)	alsC ←	D‑allose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,309,387	0	G	A	100.0%	47.2 / NA	18	T28I (ACC→ATC)	alsC	D‑allose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/5);  total (13/5)

GCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCC  >  NC_000913/4309147‑4309616                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      gCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTAttt                                                                                                                                                                                                                             >  1:280084/1‑251 (MQ=255)                         gCCAACATCAGTTTGGCGGTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGa                                                                                                                                                                                                      >  1:7730/1‑250 (MQ=255)                                        gCGGTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGACCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTctcgct                                                                                                                                                                                      >  1:126715/1‑251 (MQ=255)                                          ggTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGCACATGCCGATCAATACCGTCACGGAGCTTTGAACACAAATCTGGGTAATATTATTGGTGGTCAGCAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTctcgctcg                                                                                                                                                                                    >  1:102304/1‑251 (MQ=255)                                                             gcCAGAATCGCGCCAACCGAGAGGTAGATCCCAGCGACCAGGATAGCGAAACACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACCAAAATCTGGGTACTATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTg                                                                                                                                                                                                       >  1:269156/1‑213 (MQ=255)                                                                                              gCGACCAGGATAGCGAACAACTCGCCCATGCAGATCAATACCGTAACGGAGCTTTGAAAAACAATCTGGGTAATATTATTGGTGGACAGCAAATATTCTGGAGACAGAGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTc                                                                                                                                >  1:282272/1‑251 (MQ=255)                                                                                                         aGCGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATcgn                                                                                                                      <  1:59485/250‑2 (MQ=255)                                                                                                           cGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTACTATTATTGGTGGTCAGAAAATATTATGGTGACAGCGAACCACAGATGGCGACGATGATCGCCAGGATCAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTATTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGAt                                                                                                                   >  1:218505/1‑251 (MQ=255)                                                                                                                    ngCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTctcg                                                                                                                                                                                        >  1:35054/2‑173 (MQ=255)                                                                                                                    cGCCCATGCCGATCAATACCGTCTCGGAGCGGTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATGCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGAGAAAAAAGATGCCGTACTTAGCCCAGAACAGCTCAAAGTTGAACGGTTTTTTCCCGCTCGCGTCGCTTTTTACTCTTGGGGGAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCAGCGCGATTCGTCAgg                                                                                                           <  1:221060/250‑1 (MQ=255)                                                                                                                     gCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAACATTATTGGTGGTCAGAAAATATTATGGCGACAGCGAACCAAAGATGACGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTTTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGAt                                                                                                         >  1:242153/1‑251 (MQ=255)                                                                                                                         aTGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAACATATTCTGGTGACAGCGAACCAAAGATGGCGACGCTGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAg                                                                                                                                                                                                                      >  1:172927/1‑138 (MQ=255)                                                                                                                                                                                                          gAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTCTCCAAGAGCAGCGCAAAGTTGACCGGTTTCTTCTCGCTCGCTTCGCTTTTTCCTCTTGTGGTAATGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCCGTCGTCCTTCGc                                                                                     >  1:229182/1‑186 (MQ=255)                                                                                                                                                                                                             cnAAAGATGGCGACGATGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACgg                                                          >  1:254485/1‑210 (MQ=255)                                                                                                                                                                                                                aaGATGGCGACGAGGATCGCCAGGATAAAAAAGATGCCTTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTGCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCGCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCgg                                                                    <  1:225046/197‑1 (MQ=255)                                                                                                                                                                                                                        gacgaTGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTGCATGTAGTTCAGATGACACCATc                          <  1:116249/231‑1 (MQ=255)                                                                                                                                                                                                                         acgaTGATCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGGCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTcc     <  1:245828/251‑1 (MQ=255)                                                                                                                                                                                                                              gatCGCCAGGATAAAAAAGATGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGcc  >  1:9485/1‑249 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      GCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAGCGCCAGAATCGCGCCAACCGAGAGGTCGATACCAGCGACCAGGATAGCGAAAAACTCGCCCATGCCGATCAATACCGTCACGGAGCTTTGAACAAAAATCTGGGTAATATTATTGGTGGTCAGAAAATATTCTGGTGACAGCGAACCAAAGATGGCGACGATGATCGCCAGGATAAAAAAGGTGCCGTATTTATCCCAGAACAGCGCAAAGTTGAACGGTTTCTTCTCGCTCGCTTCGCTTTTTACTCTTGTGGTAAAGCCCATGCCATAATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCC  >  NC_000913/4309147‑4309616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑