Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	628,224	G→A	A177A (GCG→GCA)	entB →	enterobactin synthase component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	628,224	0	G	A	92.9%	25.9 / ‑4.5	14	A177A (GCG→GCA)	entB	enterobactin synthase component B
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (11/2);  total (12/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

ATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCGCTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCG  >  NC_000913/628022‑628460                                                                                                                                                                                                           |                                                                                                                                                                                                                                             aTCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGCGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACTACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATAtgtg                                                                                                                                                                                              >  1:318918/1‑251 (MQ=255)        naCGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTg                                                                                                                                                                                                                                           >  1:66505/2‑199 (MQ=255)                     gacgacACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAACTGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGCCGAGCATTTGATGTCGCTGAAATATGTGGCCgg                                                                                                                                                                                         >  1:166524/1‑236 (MQ=255)                               ttcTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCg                                                                                                                                                                                                                             >  1:310567/3‑190 (MQ=255)                                           ggCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACAGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGCTGCGCTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGACATATGTGGCCGGACGTTCTg                                                                                                                                                                                 >  1:318909/1‑222 (MQ=255)                                                      gcgTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGGATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGaa                                                                                                                                                                                                     >  1:187944/1‑191 (MQ=255)                                                                          ggAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGCTATTAAACCGTTTATGGTGGCGGATGCACTGGCCGCTTTCAGCCGTGACGAGCATTTGACGTCGCTGAAATCTGTGGCCggcc                                                                                                                                                                                       >  1:173155/1‑183 (MQ=255)                                                                                      gAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCACCCGCCGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATATGTGGCCGGACg                                                                                                                                                                                      >  1:138585/1‑174 (MQ=255)                                                                                            gagTGGACGTAACCAGCTGATTATTACCGGGGTATATGCTAACATTGGCTGTATGACCACCGCAAACGACGCATTTCTGCGCGATATTAAACAGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACAAGCATTTGATGTCGTTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGcac                                                                                                                                                 >  1:319896/1‑203 (MQ=255)                                                                                            gagTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCCCATTGGCTGTATGACAACCGCAACCGAAGCATTTATGCGCGATCTTAAACCGTTTATGGTGGCGGCTGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTgaa                                                                                                                                                             >  1:210363/1‑193 (MQ=255)                                                                                                      aaCCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGAGTGCAGCCGTGACGATCATTGGATGTCGCTTAGATATGTGGCCGGACGTTCTGGCCTGGTGGTGATGACTGAAGAATTACTGCCAGCAcc                                                                                                                                            <  1:133704/200‑1 (MQ=255)                                                                                                                                                                           cgcgATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGgc                    >  1:142874/1‑251 (MQ=255)                                                                                                                                                                            gcgATATTAAACCGTTTATGGTGGCGGATGCACTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGCAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGc                                                                                                                               >  1:43116/1‑143 (MQ=255)                                                                                                                                                                                             aTGGGGGCGGATGCACTGGCCGTTTTCAGCCGGGACGAGCATTTGATGTCGCTGAAATATGGGGCCGGACGTTCTGGCCGGGGGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGgcg  <  1:265103/251‑1 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                                             ATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAGCTGATTATTACCGGGGTATATGCCCACATTGGCTGTATGACCACCGCAACCGACGCATTTATGCGCGATATTAAACCGTTTATGGTGGCGGATGCGCTGGCCGATTTCAGCCGTGACGAGCATTTGATGTCGCTGAAATATGTGGCCGGACGTTCTGGCCGGGTGGTGATGACTGAAGAATTACTGCCAGCACCTATCCCCGCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCG  >  NC_000913/628022‑628460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑