Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,385,905 C→T L68F (CTC→TTC)  yjeO → DUF2645 domain‑containing inner membrane protein YjeO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,9050CT95.0% 52.1 / NA 21L68F (CTC→TTC) yjeODUF2645 domain‑containing inner membrane protein YjeO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/10);  minor base G (0/1);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAAT  >  NC_000913/4385673‑4386146
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
gcTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGc                                                                                                                                                                                                                                 <  1:129902/251‑1 (MQ=255)
                         tACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCGGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTATTT‑CCCTTTTTTCTgg                                                                                                                                                                                                                                              <  1:303410/213‑1 (MQ=255)
                                 gAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTGTATGTACGCCAcc                                                                                                                                                                                                 <  1:69322/250‑1 (MQ=255)
                                        ngcATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGc                                                                                                                                                                                         >  1:94115/2‑251 (MQ=255)
                                                               cTGGTTTATTGCGGCGTTCCTCTGGATAACCATCAATTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGAGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGaaaaaaa                                                                                                                                                                                                                         >  1:115123/1‑196 (MQ=255)
                                                                             cGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTgcgc                                                                                                                                                    <  1:311783/251‑1 (MQ=255)
                                                                                  ntctGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTATGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATc                                                                                                                                                >  1:96604/2‑250 (MQ=255)
                                                                                   tctGGNNAANNNTCNNTTCCGCGCTGGACAAAGAGTGGANGATTGATGGACGCGGGATTAACAACGTCTNCNANGTGTNNATGNATCTCGAAGANGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CNCNTTTTTCTGGTTCGCTTTGTGGCGAAACAAACNCGGCTGGTTTATGTACg                                                                                                                                                                                                      >  2:245501/1‑195 (MQ=255)
                                                                                   tctGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACg                                                                                                                                                                                                      <  1:245501/195‑1 (MQ=255)
                                                                                             cATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CNCTTNNNNNTgnn                                                                                                                                                                                                                                             >  2:200700/1‑144 (MQ=255)
                                                                                                         gcTGGACAAAGAGAGGATGCTTGATGCACGGGAGATTACCAACGTCTGGGATGTGTTGATGTATCTCGAAGACGACGATACTCGTGATGTCGGTGTGATAATGACGTTACGGCTCTTTTT‑CCGTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGGTGGTTTATGTACg                                                                                                                                                                                                      >  1:276741/1‑173 (MQ=255)
                                                                                                                aaaGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGAGAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTTGTTTATGTACGCCACCGCGCTGGCTATGTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATcc                                                                                                                 <  1:209022/251‑1 (MQ=255)
                                                                                                                        gatgatTGATGGACGCGGGATTAACAACGTCTGCGCTGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGtt                                                                                                                                                                                                                                            >  1:134293/1‑120 (MQ=255)
                                                                                                                                        gggATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGGTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTGTTCTGCGCTATCAGGTTTGTTTGTGAGCCGGATTGGTTCATCCGGcac                                                                                                            <  1:153674/232‑1 (MQ=255)
                                                                                                                                              aacaacGTCTGCGATGTGTTGATGTATCTCGACGAAGACGATACCCGTGATGTTGGTGCGACAATCACGCTACCGCTCTTTTTTCCCTTTT‑TCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGATTCTGTACCCAACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATTAGTTTTGTTTGTGAGCAGGATTGGTTCATCCGGCACACACACTTCCAAACTTCCCGTCTg                                                                                    >  1:286753/1‑250 (MQ=255)
                                                                                                                                                        gCGATGTGTTGATGGAGCTCGAAGAAGACGACACCCGGGATGTCGGGTTGATAATTACGCTACCGCTCTTGTT‑CCCTTTCGGCTGGCTCGCGTTGTGGCGAAAACAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTGTTCTCTGCGCTGTCAGGTTTGTTTGTGAGCCGGATTGGTTCAGCCGGCACACACACTTACAAACTTCCCGTCTGACGACCTTTg                                                                          <  1:138843/250‑1 (MQ=255)
                                                                                                                                                         cGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTTGTTCATCCGGCACTCAAACTTACAAACTTCCCGTCTGACGACCTTTGcc                                                                        <  1:258711/251‑1 (MQ=255)
                                                                                                                                                               gtTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTCCCGCTCTTTTT‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCgtct                                                                                     >  1:298218/1‑229 (MQ=255)
                                                                                                                                                                          ncGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTCCAGCTCTTTTT‑CCCTTTTTTATGGTTCGCtt                                                                                                                                                                                                                                       >  1:37913/2‑75 (MQ=255)
                                                                                                                                                                                                                      ccGCTCTTTTT‑CCCTTTTTTTTGGTTCGCTTTGTGGCGAAATAAACGCGGCTGGATTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGGCGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGGCGGGTTGGTTCATCTGGCACACACACTTATAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGACAGTGTTCTCCCCGTTTGTTTTCCGTTTCGGCTTTCCCGACGCCTCTGGa             >  1:316855/1‑249 (MQ=255)
                                                                                                                                                                                                                            ttttt‑CCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCgn      <  1:200700/250‑2 (MQ=255)
                                                                                                                                                                                                                                t‑CCCTTTTTTCTGGTTCTCTTTGTGGCTAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCATTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCTCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAAt  <  1:309657/250‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
GCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGGATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTT‑CCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAAT  >  NC_000913/4385673‑4386146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑