Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	817,542	C→T	R167C (CGT→TGT)	moaA →	GTP 3',8'‑cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	817,542	0	C	T	100.0%	21.1 / NA	11	R167C (CGT→TGT)	moaA	GTP 3',8'‑cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

GAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTG  >  NC_000913/817308‑817756                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       gAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGCAAGCGAAGTGGAGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGAGACGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAATTAACCCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACGGGGCTGATGTGTGATGTTAatcatc                                                                                                                                                                                                         >  1:222016/1‑250 (MQ=255) gAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAatcatc                                                                                                                                                                                                         >  1:222033/1‑250 (MQ=255) gAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAATTCAACCAGGGCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTAAATACCGTGCTGATGTGGGATGTTAatcatc                                                                                                                                                                                                         >  1:222017/1‑250 (MQ=255)  aaaaCGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACAGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCAc                                                                                                                                                                                                       >  1:275023/1‑251 (MQ=255)                         gCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCTGGACTTACTGGCATTAACGTCAGTGGCGACAGTCGGGACGCCCGCCATTTTCACGCTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGTGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTg                                                                                                                                                                                <  1:55743/251‑1 (MQ=255)                                                             gtgGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGAAAGTCTGGACGCCCGCCAGTTTCACTCTATTACCGGGCAGGAGAAATTCAACCAGGTCATGGCAGTGATTGATGCTGCATTTGAGGCCGTTTTTGAGAAGGTCAAATTCAATACCGTGCTTATGTGTGATGTTAATCATCACCAGCTCGACACCTTTCTGa                                                                                                                                                                                    <  1:50682/211‑1 (MQ=255)                                                                                                        gtgtCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGTGCAGGATAAATTCAACCAGGGCATGGCAGGGATTGAGGCGGCATTTGAGGCCGGTTTTGAGAATGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGa                                                                                                                                                                                               <  1:146325/157‑1 (MQ=255)                                                                                                                                                  ggCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTcgn                                                       <  1:80553/251‑2 (MQ=255)                                                                                                                                                                      gTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCAGCTTCGTTTCATCGAACGGATGGAAACGGACGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTc                                                                                    <  1:175452/202‑1 (MQ=255)                                                                                                                                                                              ngggATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTg                                                  >  1:193889/2‑228 (MQ=255)                                                                                                                                                                                                          tttGAGAAGGTCAAAGTCAATACCGTGCTGATGTGTGATGTTAATCATCACCAGCTCGACACCTGTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCGGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTg  <  1:277843/248‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       GAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAAGTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTG  >  NC_000913/817308‑817756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑