Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 757,445 C→T C513C (TGC→TGT)  sdhA → succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913757,4450CT90.0% 19.0 / ‑3.6 11C513C (TGC→TGT) sdhAsuccinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (8/1);  total (9/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

GCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAT  >  NC_000913/757234‑757690
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
gCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGACGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCACCACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACg                                                                                                                                                                                                                >  1:216619/1‑251 (MQ=255)
 ccTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGAAAGAATGTATGAAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACgg                                                                                                                                                                                                               >  1:242787/1‑251 (MQ=255)
                                            gCGATACGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTTAATGTGATGCGATGGCGAAAGGGCTTGAGCAGGTGAAAGTGATCCGCGTGCGTCTGAAAAATGCCCGTCTGGATGACACGTCCAGCGAGTTCAACACCCAGCGCGTGTAGGGGCTTGAACTGGAGAACCTGATGGAGACTGCGTATGCAACGGCTGTTGGTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATan                                                                                                                                                                    <  1:37906/251‑2 (MQ=255)
                                                  cGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCtt                                                                                                                                                              >  1:121538/1‑251 (MQ=255)
                                                                                                           gCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAACATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGtgatgatga                                                                                                                                      >  1:115078/1‑218 (MQ=255)
                                                                                                                  cGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGcc                                                                                                        <  1:19640/241‑1 (MQ=255)
                                                                                                                        ggCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACACAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGc                                                                                                                         >  1:185986/1‑218 (MQ=255)
                                                                                                                                         gTGATCCGCGAGCGTCTGACAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAACCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCa                                                                        >  1:267113/1‑250 (MQ=255)
                                                                                                                                           gATCCGCGAGCGTCTGAAAAATGCCCGTATGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGCTAACCTGATGGAAACAGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGGGCATAGCCg                                                                                                                                                                 >  1:27072/1‑159 (MQ=255)
                                                                                                                                                                                        cGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATACCCTGATGGAAACGGCGTCTGCAACGGCTGTTTCTGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGCACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt                        >  1:172484/1‑251 (MQ=255)
                                                                                                                                                                                                              gAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGAATAGCCGCTTCGATTTACAGGACCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCTAGAGTCGGAATCCATGACGCGCCGAAGAGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt  >  1:326765/1‑251 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
GCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAT  >  NC_000913/757234‑757690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑