Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,037	G→A	A97V (GCC→GTC)	fumB ←	fumarase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,037	0	G	A	100.0%	27.9 / NA	12	A97V (GCC→GTC)	fumB	fumarase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

CGATTTGCGCAGGCAGGTTAGTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGG  >  NC_000913/4346822‑4347271                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           cGATTTGCGCAGGCAGGTTAGTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTACGAATTGCAGCGCCACg                                                                                                                                                                                                         >  1:5847/1‑251 (MQ=255)       gcgcAGGCAGGTTAGTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTCCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTg                                                                                                                                                                                                   >  1:20341/1‑251 (MQ=255)                         cGGTGTTGCCCTCTTTGTACATGTCCAGCGCCGCATTCTGTGCATAGCGCAGGTTCTCATCGCTATAGGTGTTATAGACGCCTTTCGCCAGCGTTTATTCATCAGTGCCGCCGGTACACACGCGGTGACCTTTTTTACCGACGATGCTCGCGGGGCAGGTATCATGGCCGGTCGGCAGCCAGCCTTTGGCGACGGTTTCGGAGTTTCTTACGAATTGCCACGCCACGTACTTGTCGTTTTCGCTGGCTTCt                                                                                                                                                                                 >  1:48989/1‑251 (MQ=255)                          ngtgtTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTCTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTg                                                                                                                                                                                >  1:58365/2‑251 (MQ=255)                                                                                                  ataGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTGTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTc                                                                                                                                                                                                                             <  1:136412/134‑1 (MQ=255)                                                                                                                         cttcATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCt                                                                                 >  1:21420/1‑251 (MQ=255)                                                                                                                         cttaatcaCCGCCGCCGGTCCACACGCGATGGCCTTTTTTACCGACGATGATCGCGGCGACGGTATCCTGGCAGGTGGGCAGCACGGCTTTGGTGAAGATTTCGGCGTTTCTCCAGAATTGCAGCGCCAAGTACTTGACGCTTTCCCTGGCTTCTGGATAGCGCAGAATAGCCg                                                                                                                                                              >  1:190160/5‑174 (MQ=255)                                                                                                                                                                                    ccGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGc                                                                                                                                      <  1:20476/139‑1 (MQ=255)                                                                                                                                                                                    ccGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCNNGCTTCt                                                                                                                                                                                 >  2:20476/1‑96 (MQ=255)                                                                                                                                                                                      ggTATCCTGGCAGGTCGGCAGCACGCCATTGGGGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATGGTGAAGAATAGCCGCAACCTGTTTCGGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCca                                                                                               <  1:322924/176‑1 (MQ=255)                                                                                                                                                                                                   gTCGGCAGCACGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCg                                                   <  1:276467/207‑1 (MQ=255)                                                                                                                                                                                                        cagcaCGCCTTTGGCGACGATTTCGGAGTTTCTTAAGAATTGCAGCGCAACGTACTTGTAGTTTTCGCTGGCTTATGGATAGTGAAGAATAACCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGATGCGCCAGCCGGGTCGGGGCTTCTGGTTCGACTATCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTCATAGGAAGTGAGTAGATAGTATTCgg  >  1:228204/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           CGATTTGCGCAGGCAGGTTAGTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGG  >  NC_000913/4346822‑4347271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑