Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,864,407	C→T	L58L (CTG→TTG)	ygbM →	putative 2‑oxo‑tetronate isomerase YgbM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,864,407	0	C	T	100.0%	24.3 / NA	11	L58L (CTG→TTG)	ygbM	putative 2‑oxo‑tetronate isomerase YgbM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GACCGCAGGTGAAATTGCGGAATTAAGGAGTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTGCCCGCTGGCGAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGC  >  NC_000913/2864198‑2864631                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 gACCGCAGGTGAAATTGCGGAATTAAGGAGTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATgg                                                                                                                                                                                         >  1:7879/1‑251 (MQ=255)  aCCGCAGGTGAAATTGCGGAATTAAGGAGTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATggn                                                                                                                                                                                        <  1:63714/251‑2 (MQ=255)          tGAAATTGCGGAATTAAGGAGTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTCCTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGga                                                                                                                                                                                                              <  1:19993/221‑1 (MQ=255)                              gTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCGATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATTTGACACTGGCGTTGTTCAATGCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGa                                                                                                                                                                    <  1:146457/243‑1 (MQ=255)                                              gTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTACTCCACCATGCAAATCCAACAGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt                                                                                                                                                                                     >  1:284185/1‑210 (MQ=255)                                                                                                                                                        taACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcac                                                                                                                                                    >  1:136689/1‑137 (MQ=255)                                                                                                                                                                               aaaaGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTGCCCGCTGGCGAAGATGCTGAACGGTAccn                                          <  1:198375/220‑2 (MQ=255)                                                                                                                                                                               aaaaGCAACTGGAGCAAAACCATCTGACAATGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCNCTGNNNNNTgnnnnnnnnnn                                                                                                            >  2:198375/1‑144 (MQ=255)                                                                                                                                                                                 aaGCAACTGGAGCAAAACCATCTGACACTGGCGTTGTTCAATTCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTGCCCGCTGGCGAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGc         <  1:234072/251‑1 (MQ=255)                                                                                                                                                                                         ggAGCAAAACCATCTGACACTGGCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTGCCCGCTGGCGAAGATGCTGAACGGTATCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGc  <  1:75910/250‑1 (MQ=255)                                                                                                                                                                                          gAGCAAAACCATCTGACACTGTCGTTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATTGGGATTATCCGCCCTGCCCGTACGTGAGCGCGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTg                                                                       <  1:280400/180‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 GACCGCAGGTGAAATTGCGGAATTAAGGAGTTAATGCAATGCCTCGTTTTGCAGCTAATTTATCCATGATGTTCACCGAAGTGCCTTTTATTGAACGCTTCGCCGCAGCGCGAAAAGCCGGGTTCGATGCTGTGGAATTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGGCGCTCAATTGTGAACAAGTCCATGTGATGGCAGGCGTCGTGCCCGCTGGCGAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGC  >  NC_000913/2864198‑2864631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑