Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	680,493	G→A	Q94Q (CAG→CAA)	djlC →	co‑chaperone DjlC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	680,493	0	G	A	93.8%	39.4 / ‑3.8	16	Q94Q (CAG→CAA)	djlC	co‑chaperone DjlC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/7);  minor base C (1/0);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

TGCTCGCTTACCGTTGTGTCATCCCGAAACCGATCCGCAAGGGTTTAAAGCATTACGCCAGGCCTATGAAGAGGCCCTGCGACTGGCGGTAAATCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAGAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTCAGGCTGG  >  NC_000913/680283‑680699                                                                                                                                                                                                                   |                                                                                                                                                                                                               tGCTCGCTTACCGTTGTGTCATCCCGAAACCGATCCGCAAGGGTTTAAAGCATTGCGCCAGGCCTATGAAGAGGCCCTGCGACTGGCGGTAAATCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGAt                                                                                                                                                                         <  1:307850/250‑1 (MQ=255)                                   ncGCAAGGGTTTAAAGCATTACGCCAGGCCTATGAAGAGGCCCTGCGACTGGCGGTAAATACTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGc                                                                                                                                      >  1:36723/2‑251 (MQ=255)                                                gacaaatccaccAGGCCTCTGAAGAGGCCCTGCGACTGGCGGTAAATCCTGTCGAGGAAGCAGATGCTGAAGAAAAAGCTGCTGCCGCTGCACATGAAATACTACGTGCCTTCAGGACATTACTGGATTCAGACAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATAACTGGAACCTGGAGGATGTCGATCAATTACGCTGGCCGCtgtgtg                                                                                                                                             >  1:301754/11‑231 (MQ=255)                                                                cTATGAAGAGGCCCTGCGACTGGCGGTAAATCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCAGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGCTCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAAtt                                                                                                         >  1:147578/1‑251 (MQ=255)                                                                                     ggCGGTAAATCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAAGTGTGCTTCTTTGATGGCagag                                                                                     <  1:249745/250‑1 (MQ=255)                                                                                             aTCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTc                                                                                                                                                                                                      <  1:9204/129‑1 (MQ=255)                                                                                                                      agaaAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATGCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATGAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAg                                                   <  1:185818/251‑1 (MQ=255)                                                                                                                                    ncGCTGAACATGAAATACTACGTGCATTCAGGACATTCCTGGATTCAGAAAGTGATCGTTTTCAGCCTTAAGCCTGGCAAAAATTTATTc                                                                                                                                                                                                      >  1:60756/2‑90 (MQ=255)                                                                                                                                                     ctacGTGCATTCAGGACATTACTGGATTCAGAAAGTGATAGTTTTCAGCTTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGCGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATtg                                                                                                        >  1:282744/1‑167 (MQ=255)                                                                                                                                                        ngTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGCGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTg                  >  1:94772/2‑250 (MQ=255)                                                                                                                                                                cAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGGTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGATGCTGTTCTTGATGCCATTc         <  1:260050/251‑1 (MQ=255)                                                                                                                                                                  ggACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTACGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTc         >  1:215982/1‑249 (MQ=255)                                                                                                                                                                         aCTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACAGGGAGGATGTCGATCAATTACGCTGGCCGCtgtgtg                                                                                                                                             <  1:268711/110‑1 (MQ=255)                                                                                                                                                                                   gAAAGGGATCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTCAGGCTgg  >  1:279032/1‑239 (MQ=255)                                                                                                                                                                                          aTCGTTTTCAGCCTTCCGCCTGGCAAAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGc              <  1:127708/220‑1 (MQ=255)                                                                                                                                                                                          aTCGTNTTCAGNCNNCCGCCTGGCACAAATTTATNCAGCAATTAAATACCNGGAANATggangtngn                                                                                                                                                                       >  2:127708/1‑63 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                               TGCTCGCTTACCGTTGTGTCATCCCGAAACCGATCCGCAAGGGTTTAAAGCATTACGCCAGGCCTATGAAGAGGCCCTGCGACTGGCGGTAAATCCTGTCGAGGAAGCAGATGATGAAGAAAAAGATGCTGCCGCTGAACATGAAATACTACGTGCATTCAGGACATTACTGGATTCAGAAAGTGATCGTTTTCAGCCTTCCGCCTGGCAGAAATTTATTCAGCAATTAAATACCTGGAACATGGAGGATGTCGATCAATTACGCTGGCCGCTGTGTGCAATCGCCATAGAAGCGCGATATCTTTCATTAAATTGTGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTCAGGCTGG  >  NC_000913/680283‑680699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑