Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	11.3 / NA	6	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/3);  total (3/3)

GGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTC  >  NC_000913/1337308‑1337633                                                                                       |                                                                                                                                                                                                                                                ggTTTAACCTTTTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGTGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGTCAACCGTAACTTTTAAGGCCGTATCCATCCGCTGGTTAAAACTAACGGGCTTGCCTCGCCGCCTCTTGTGGTTTCCTATGCGCTGGCTGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAt                                                                             <  1:278969/251‑1 (MQ=255)                 aTACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTACGGCaan                                                                                                                                                                                                              <  1:78124/106‑2 (MQ=255)                                        gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCCTGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGcc                                       >  1:147853/1‑250 (MQ=255)                                                                       aaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGTCGGGAAATATGAATATCAACCTGGCTGCTGAGCCTATCGGCCATGAGCGCAAAGGCGATTCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAAc       <  1:307817/251‑1 (MQ=255)                                                                            cAATCAAAACAGGCGATTTACCCGACGGTGCGGTGCTGTCCGGCAACCGTAACTTTGATGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGCCCTCGCCGCCGCTGGTGGTTGCCTACGCGCTGGCGGAAAATATGACTATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCAGGTTTATCTGAAAGACATCTGGCCCTCGGCCAAAGAAATTGCCAGTGCGGGAGAACAAGtc  >  1:266504/1‑251 (MQ=255)                                                                            cAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTg                      >  1:98451/1‑231 (MQ=255)                                                                                       |                                                                                                                                                                                                                                                GGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTC  >  NC_000913/1337308‑1337633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑