Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,995	C→T	L113L (CTG→TTG)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,995	0	C	T	88.9%	16.2 / NA	9	L113L (CTG→TTG)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/3);  minor base G (0/1);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTG  >  NC_000913/1726755‑1727192                                                                                                                                                                                                                                                 |                                                                                                                                                                                                      gAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCACGGCAACCGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACAGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCCCGCCAGATTGCAACCTgg                                                                                                                                                                                             >  1:208179/1‑251 (MQ=255)                                    aTACTATCGCCAACGTGCCATTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAACAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCGGTGGCACACCGGACGCATTTCTCATGCCAGCTTGCATCCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAgag                                                                                                                                                          <  1:313580/251‑3 (MQ=255)                                                                                                                                              cAAATTGCCGCATGGAAAAAAATNACCGCTGGCGNTCANNCTGANANTNGTCATATGGCCGTGCAGCTGTGGCACACCNGACGCATTTCTCACGCCAGCTTGNNACNNNGCNNTCAgg                                                                                                                                                                                     <  2:240122/118‑1 (MQ=255)                                                                                                                                              cAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATCTGGCCGTGCAGCTGTGCCACACCGGACGCATTTCTCACGCCAGCTTGCAACCTGGCGGTCAgg                                                                                                                                                                                     >  1:240122/1‑118 (MQ=255)                                                                                                                                              cAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCTTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGCCCACGTACTTCTCTGCGCGATGACAATGGTCAGGCGCTCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCCGGTATCGTCAATg                                                 >  1:296683/1‑250 (MQ=255)                                                                                                                                                           ggAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCTTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGAGCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGc                                    <  1:123572/250‑1 (MQ=255)                                                                                                                                                                         ncTGGCGTTCCTGCTGAAAATGGTCATATGGCCGTGCACCCGGGGCACACACGACGCATTTCTCACGCCAGCTTGCAACCTGGCGGGTAGCCACCGGTAGCGCCTTTCGCTCTTAGCGCGGGAACACGCACTTCTCTGCGCGATGAAAATGGGCCGGCGATCCGGGTTGAAACATCCATCCCGCCGGCGCCTTACCTGCGAGCGATTCCAGGGCTCGTAAATGATTTCCGTCAGGCCATTGTTAAcgcg                       >  1:63134/2‑249 (MQ=255)                                                                                                                                                                                                                  ntgGCACACCGGACGCATTTCTCACGCCAGCTTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTg  >  1:195991/2‑229 (MQ=255)                                                                                                                                                                                                                    ggCACACCGGACGCATTTCTCACGCCAGCGTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCTCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGc                              <  1:154417/199‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                      GAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTG  >  NC_000913/1726755‑1727192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑