Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,505,212	C→T	S270N (AGC→AAC)	yhfS ←	putative aminotransferase YhfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,505,212	0	C	T	91.7%	24.3 / NA	12	S270N (AGC→AAC)	yhfS	putative aminotransferase YhfS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (3/8);  minor base G (0/1);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCA  >  NC_000913/3504981‑3505409                                                                                                                                                                                                                                        |                                                                                                                                                                                                      tCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGgcac                                                                                                                                                                                       <  1:77090/248‑1 (MQ=255)                        gCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTGCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAGCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGGTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAgn                                                                                                                                                              <  1:63165/250‑2 (MQ=255)                                                                  cGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGgcg                                                                                                                   <  1:311110/251‑1 (MQ=255)                                                                                                     cATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCTACTGCGCATTAGCAATCACCGCGTTTTTCAc                                                                                                                                                                                                <  1:117867/139‑1 (MQ=255)                                                                                                                     ccccaccGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGc                                                                <  1:284204/250‑1 (MQ=255)                                                                                                                     acccaccGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCATCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGc                                                                <  1:162871/251‑1 (MQ=255)                                                                                                                                            cgcgCTTTTGCGCCTCGTCCAGCACTCTGTCGGCAATCTGCTGATGAAACTCGACAATCAACTCCTTCTACTGCGCATTAGCAATCTCCGCGTTTTTCACTTCCGTCACCGCACCACCGTTAAGCAAACCCAGCAACCTTTTAGAAACCCCTGTCTGCTCCGCGTGCATCACGGGCTCAAACACCAGACCACGCAATACTTCCAGCGCCCTTGTGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGgcg                                         <  1:310506/251‑1 (MQ=255)                                                                                                                                                              ccAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGTCTACCGCCGGAGTAAAGCGGGGCGCTAATACGGTTGATAAc                        <  1:260783/250‑1 (MQ=255)                                                                                                                                                                   cctctGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCGGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGGTGATAACATCAGc                  <  1:265962/250‑1 (MQ=255)                                                                                                                                                                                   gCTGATGANNCTNNANANTCAACNCCTTCGACTGCGCATTAGCAATCACCGCGTTTTTCACTTCCg                                                                                                                                                                                           >  2:211678/1‑66 (MQ=255)                                                                                                                                                                                                                    gcgcATTAGCAATCACCGCGTTTTTCACTTCCGGCACCGCCCCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGCTAACATCAGCATCGCCGAcc        >  1:278528/1‑212 (MQ=255)                                                                                                                                                                                                                             cAATCACCGCGTTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCa  >  1:189000/1‑209 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                      TCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCA  >  NC_000913/3504981‑3505409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑