Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	92.3%	30.6 / NA	13	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (5/7);  minor base C (1/0);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGC  >  NC_000913/2397272‑2397575                                                                                                                                                                             |                                                                                                                                     cGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAc                                                       <  1:106578/251‑1 (MQ=255)   aaCCGCCCCAGCCAACACGGTTAGATAAGTAACGGATCGGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAAAAAACCGCAGGATCACCACCGCTTTGCGGATGGTCAGCAGGATATCAATCAGTTCCGGTGAGATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACAAACCCATCGGCACGCCCAcct                                                     >  1:207497/1‑251 (MQ=255)                           nnnnnnnaNGGNNCTGANACAGACCCAGCAGGCGACGTTCGCNAAAGCGCATGAAGGCCCCGTAGGTGAAAACCNCNAGCAGGATCGCCACCGCTTAGAGGATGGTCAGCAGGATCGCAATCAGTNCCTGTGATATNCAANNCATGNTNGNGCCCCCTCTG‑GATCCTCAAGAAGCGCGCCNGCCAGCACCGGAGCAATGCCGTANNTANNNATNNGCAAg                                                            <  2:244594/213‑1 (MQ=255)                             ccGTAACGGTTCTGGAACANACCCAGCGGGCGACGTTCGCCAAAGGGCAGGNANGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGNTCAGCAGGATNTCANNCAGTNCNGNTGATATCCAACTCATTATAGTGCCTCCT‑TNAGATCCTCAAGATGCGCGCCAGCNNGCNNNGGNNCAATg                                                                                 <  2:236117/197‑1 (MQ=255)                             ccGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGATGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATAGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATg                                                                                 >  1:236117/1‑197 (MQ=255)                                                naCCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCg        >  1:82785/2‑251 (MQ=255)                                                gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGcn                                                          <  1:200589/201‑2 (MQ=255)                                                gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCNCCT‑NNNNNTcnn                                                                                                                 >  2:200589/1‑144 (MQ=255)                                                gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGATCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATTGGAAAGCCCACCTGCCCTGCCGTCAGTCCTTCGACGATTTCAACCGGCAGCGTGACCg        >  1:231218/1‑251 (MQ=255)                                                      gcagGCGACGTTCGCCAAAGCTCATGTATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAGCAGTTCCGGTGATATCCAACTCAGTATTGTGCCTCCT‑GGAGATCCTCAAGATGCGCGCCAGCCAGCAGCGGAGCAATGCCGGACATACCCAGCGGCAAGCCTACCTGCCCTGCCGGCAGTCCTTCGGCGATTTCCACCGGCAGCGTGACCGTGTTGc  <  1:213196/251‑1 (MQ=255)                                                                 tCGCCAAAGCTCATGAATGCCACGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCACAATCAGATCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCGGCCCTGCCGGCAGTCCTTCGGCGATTTCAACCGGCAGTGTGACCgggt     <  1:117169/237‑4 (MQ=255)                                                                                   gCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCan                <  1:84524/208‑2 (MQ=255)                                                                                           ggTGACAACCACCAGCAGGATCACAACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGAGATATCCAAATCATTCTTGTGCCTCCT‑TGCGATCATCAAGATGCGCGCCAGCCAGCACCGGAGCACTGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGc                  >  1:159835/1‑198 (MQ=255)                                                                                                                                   gTCAGCAGGATCTCAATGAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCT‑TGAGATCCTCAAGAGTCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgccc                                                 <  1:280952/127‑1 (MQ=255)                                                                                                                                                                             |                                                                                                                                     CGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCT‑TGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGC  >  NC_000913/2397272‑2397575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑