Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,878	C→T	G92D (GGC→GAC)	btuC ←	vitamin B12 ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,878	0	C	T	88.9%	16.0 / ‑6.1	9	G92D (GGC→GAC)	btuC	vitamin B12 ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/3);  minor base A (1/0);  total (6/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCT  >  NC_000913/1794682‑1795065                                                                                                                                                                                                     |                                                                                                                                                                                            gcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGAAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAgcgc                                                                                                                                       >  1:181179/1‑251 (MQ=255)                          ccagccagCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCGGCGCCGTTAGAGACGTCAAGTAGTCCAGGTGCTGCCAGAGGATTTGCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCaccaac                                                                                                              <  1:214415/251‑1 (MQ=255)                                       aCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGAAATAGCAAACAGCCCTCGCGACAAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCCGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAcagac                                                                                                 >  1:234750/1‑249 (MQ=255)                                                 ngAAAGATGACGACGGGCGAAACGTAAGCGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGcgccg                                                                                                                     >  1:82895/2‑221 (MQ=255)                                                             acgGGCGAAACGTAAGAGTATTAAAATGGTGCCAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGACCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCac                                                                                                                  >  1:100849/1‑212 (MQ=255)                                                                ggCGAAACGTAAGAGTATAAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAgc                                                                                                                                         <  1:110198/186‑1 (MQ=255)                                                                                                        gCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAggn                                                                                 <  1:37026/202‑2 (MQ=255)                                                                                                                       ccTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGTCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTACCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTc                 >  1:27324/1‑251 (MQ=255)                                                                                                                                             gcccttgcccAAGCAATACCGCGGCGATAAGCCTCACGCCTGCGCCGTTAGAGACGACAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACCGCGCCGGATATAGCCAGCGCCGCCCCAACCAGCACTACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGCGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCt  >  1:163914/1‑244 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                            GCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCT  >  NC_000913/1794682‑1795065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑