Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,344,255	G→A	R296H (CGC→CAC)	melB →	melibiose:H(+)/Na(+)/Li(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,344,255	0	G	A	100.0%	33.1 / NA	14	R296H (CGC→CAC)	melB	melibiose:H(+)/Na(+)/Li(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

CCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTG  >  NC_000913/4344016‑4344448                                                                                                                                                                                                                                                |                                                                                                                                                                                                  ccATCTGACACTTAAAGCCATCGTTGAGCTAATTTATACACACGATCAGCTTTCATGCCTCTTGGGTATGACTCTTGCTTATAATGTCGCCCGCAACATTATTACCGGCTTTACTATCTATTCTTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGAGACGTTAGAATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATggg                                                                                                                                                                                        >  1:50247/1‑251 (MQ=255)        acacTTAAAGCCATCGTTGCGCTAATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCGTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTTGTATGCGGGAGCTGCGAACCTGGTGACGTTAGTATGCTGCCCACGCTTAGTTAAATCATTATCCCGCCACATTTTATGGGCCGGAGc                                                                                                                                                                                 <  1:127624/251‑1 (MQ=255)                      cGTTGCGCTAATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTGGTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTAGTATCTGGCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATGGGCCGGAGCATCTATTCTTCCg                                                                                                                                                                    <  1:331034/250‑1 (MQ=255)                                                                                      gTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGCTGACGCGGATTTCTTCCCCTCTTATCTGTCGTCTCCGGGCACTGCTACCCTGCTGACGTCCGTATTCTTCCCACGCTTAGTTAAATCATTATCCAGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTTTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATtgt                                                                                                   >  1:226206/1‑251 (MQ=255)                                                                                           cAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATGTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTGCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGAttn                                                                                              <  1:36542/251‑2 (MQ=255)                                                                                                             ntttGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGCGTTAAGCTGTGGTGTTCTCCTGTTCATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAAtg                                                                            >  1:27779/2‑251 (MQ=255)                                                                                                                 gCTATCTATTATTTCTCATATGTTATCTGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTTACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTTTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTggg                                                                        <  1:213593/251‑1 (MQ=255)                                                                                                                                 cATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATGTTATGGGCCGGAGCATCTATTCTTCCGTGGTTAAGCGTTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACATCGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCt                                                          <  1:324650/249‑1 (MQ=255)                                                                                                                                               gATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATGATCCCGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGTGCTATCACAAc                                                                                                                 <  1:256747/180‑1 (MQ=255)                                                                                                                                                               ccTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCACACGCTTAGTTAAATCATTATCGCGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCATCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGATTTTCTGGGTATTAc                                                 >  1:165155/1‑228 (MQ=255)                                                                                                                                                                                 cGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAACTCTTTACCCAGACACCTTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACACCGTCGTCCTCATTGTGCTTGCGGGTATTTTGCTGACTGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAatat        >  1:181935/1‑251 (MQ=255)                                                                                                                                                                                                 ggTGACGTTAGTATTCTTCCCACGCTTAGTTAAAGCATTATCCCTACACATTTTATGGGCCGGAGCATCTATGCTTCCGGTGTTAAGCTGTTGTGTGCTCCTGTTAATGGCATTAATGAGCTATCACAAc                                                                                                                 <  1:285651/130‑1 (MQ=255)                                                                                                                                                                                                      ngTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTg  >  1:196938/2‑236 (MQ=255)                                                                                                                                                                                                             aTTCTTCCCACGCTTAGTTAAATCATTATCCCGACACATTTTATGGGCCGGAGCAGCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCGCATTGGGAttn                                                                                              <  1:261683/137‑2 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                  CCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTG  >  NC_000913/4344016‑4344448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑