breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 178,317 C→T S49N (AGC→AAC)  btuF ← vitamin B12 ABC transporter periplasmic binding protein
RA 178,334 C→T T43T (ACG→ACA btuF ← vitamin B12 ABC transporter periplasmic binding protein
RA 178,631 C→T A175T (GCG→ACG)  mtn ← 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
RA 200,942 C→T A154V (GCC→GTC)  skp → periplasmic chaperone Skp
RA 204,796 C→T P102S (CCG→TCG)  rnhB → RNase HII
RA 205,776 C→T V217V (GTC→GTT dnaE → DNA polymerase III subunit alpha
RA 212,279 C→T intergenic (+13/‑52) yaeR → / → tilS VOC domain‑containing protein YaeR/tRNA(Ile)‑lysidine synthetase
RA 216,901 C→T V35I (GTA→ATA)  yaeF ← peptidase C92 family protein YaeF
RA 217,760 C→T G339D (GGT→GAT)  proS ← proline‑‑tRNA ligase
RA 221,249 C→T A125T (GCA→ACA)  metI ← L‑methionine/D‑methionine ABC transporter membrane subunit
RA 231,185 C→T P22S (CCG→TCG)  yafD → endonuclease/exonuclease/phosphatase domain‑containing protein YafD
RA 233,579 C→T S126N (AGC→AAC)  mltD ← membrane‑bound lytic murein transglycosylase D
RA 242,070 C→T G412R (GGG→AGG)  fadE ← acyl‑CoA dehydrogenase
RA 248,882 C→T intergenic (+748/‑2016) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 249,083 C→T intergenic (+949/‑1815) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 250,169 C→T intergenic (+2035/‑729) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 254,733 C→T P328P (CCG→CCA pepD ← peptidase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 260,454 C→T L23L (CTG→TTG)  proB → glutamate 5‑kinase
RA 265,722 C→T Q122Q (CAG→CAA ykfB ← protein YkfB
RA 273,075 C→T L77F (CTC→TTC)  ykfC → PF00078 domain‑containing protein YkfC
RA 278,949 C→T intergenic (‑147/+229) afuC ← / ← insB2 ABC transporter ATP‑binding protein AfuC/IS1 family protein InsB
RA 281,174 C→T L270L (CTG→CTA yagA ← integrase core domain‑containing protein YagA
RA 282,343 C→T G22G (GGC→GGT yagE → putative 2‑dehydro‑3‑deoxygluconate aldolase
RA 295,643 C→T intergenic (‑64/+53) yagN ← / ← intF protein YagN/putative phage integrase
RA 295,941 C→T V386M (GTG→ATG)  intF ← putative phage integrase
RA 298,492 C→T E79K (GAG→AAG)  paoD ← molybdenum cofactor insertion chaperone PaoD
RA 307,380 C→T W651* (TGG→TGA ecpC ← putative fimbrial usher protein EcpC
RA 311,285 C→T E18K (GAA→AAA)  matA ← DNA‑binding transcriptional dual regulator MatA
RA 313,256 C→T intergenic (‑14/‑2035) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 323,608 C→T A424V (GCT→GTT)  ykgF → putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
RA 325,124 C→T D81N (GAT→AAT)  ykgH ← uncharacterized protein YkgH
RA 326,430 C→T G273D (GGC→GAC)  betA ← choline dehydrogenase
RA 326,658 C→T R197H (CGC→CAC)  betA ← choline dehydrogenase
RA 336,481 C→T A186V (GCG→GTG)  yahE → DUF2877 domain‑containing protein YahE
RA 337,558 C→T P261S (CCT→TCT)  yahF → putative acyl‑CoA synthetase YahF
RA 338,812 C→T A163V (GCC→GTC)  yahG → DUF1116 domain‑containing protein YahG
RA 345,027 C→T intergenic (+36/‑377) yahL → / → yahM uncharacterized protein YahL/uncharacterized protein YahM
RA 345,487 C→T G28G (GGC→GGT yahM → uncharacterized protein YahM
RA 353,712 C→T A336V (GCC→GTC)  prpE → propionyl‑CoA synthetase
RA 370,679 C→T P135S (CCT→TCT)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase
RA 375,134 C→T intergenic (+253/‑325) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 377,177 C→T P136S (CCC→TCC)  yaiL → DUF2058 domain‑containing protein YaiL
RA 377,411 C→T intergenic (+100/+124) yaiL → / ← frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
RA 378,365 C→T E2K (GAA→AAA)  frmB ← S‑formylglutathione hydrolase FrmB
RA 382,629 C→T pseudogene (244/282 nt) yaiX ← putative acyltransferase, N‑terminal fragment
RA 383,082 C→T G285D (GGC→GAC)  yaiP ← putative glucosyltransferase
RA 383,736 C→T G67D (GGC→GAC)  yaiP ← putative glucosyltransferase
RA 387,367 C→T L133L (CTG→TTG)  tauC → taurine ABC transporter membrane subunit
RA 388,832 C→T G299D (GGT→GAT)  hemB ← porphobilinogen synthase
RA 400,881 C→T A15T (GCG→ACG)  ddlA ← D‑alanine‑‑D‑alanine ligase A
RA 422,905 C→T P130S (CCT→TCT)  malZ → maltodextrin glucosidase
RA 438,773 C→T D469N (GAT→AAT)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 440,384 C→T G240S (GGT→AGT)  ispA ← geranyl diphosphate/farnesyl diphosphate synthase
RA 441,479 C→T intergenic (‑136/‑70) xseB ← / → thiI exodeoxyribonuclease VII subunit XseB/tRNA uridine 4‑sulfurtransferase
RA 502,376 G→A S288F (TCC→TTC)  ybaL ← putative transporter YbaL
RA 505,321 G→A P136P (CCG→CCA ushA → 5'‑nucleotidase/UDP‑sugar hydrolase
RA 506,266 G→A A451A (GCG→GCA ushA → 5'‑nucleotidase/UDP‑sugar hydrolase
RA 510,440 G→A L314L (CTG→TTG)  copA ← Cu(+) exporting P‑type ATPase
RA 511,514 G→A intergenic (‑135/‑127) copA ← / → glsA Cu(+) exporting P‑type ATPase/glutaminase 1
RA 518,196 G→A F28F (TTC→TTT cnoX ← chaperedoxin
RA 520,639 G→A G75D (GGC→GAC)  ybbP → putative ABC transporter membrane subunit YbbP
RA 522,844 G→A intergenic (+14/‑417) ybbP → / → rhsD putative ABC transporter membrane subunit YbbP/protein RhsD
RA 530,193 G→A A345V (GCG→GTG)  selU ← tRNA 2‑selenouridine synthase
RA 530,540 G→A C229C (TGC→TGT selU ← tRNA 2‑selenouridine synthase
RA 534,609 G→A V232I (GTT→ATT)  gcl → glyoxylate carboligase
RA 538,791 G→A V387I (GTA→ATA)  ybbW → putative allantoin transporter
RA 540,612 G→A Q16Q (CAG→CAA ybbY → putative purine transporter
RA 540,923 G→A S120N (AGC→AAC)  ybbY → putative purine transporter
RA 542,098 G→A E71K (GAA→AAA)  glxK → glycerate 2‑kinase 2
RA 548,778 G→A G141D (GGC→GAC)  ylbE → DUF1116 domain‑containing protein YlbE
RA 549,424 G→A W356* (TGG→TGA ylbE → DUF1116 domain‑containing protein YlbE
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 623,386 G→A G363S (GGC→AGC)  entS → enterobactin exporter EntS
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 642,474 T→C Y129Y (TAT→TAC ybdR → putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
RA 643,399 G→A I7I (ATC→ATT yldA ← protein YldA
RA 647,057 G→A G151G (GGC→GGT citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 665,500 G→A L272L (CTG→TTG)  mrdB ← peptidoglycan glycosyltransferase MrdB
RA 683,107 G→A S96F (TCT→TTT)  hscC ← chaperone protein HscC
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,214,513 C→T Q321Q (CAG→CAA bluF ← blue light‑ and temperature‑regulated antirepressor BluF
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,314,350 G→A G111S (GGT→AGT)  ompW → outer membrane protein W
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,443,340 C→T A97V (GCT→GTT)  ydbH → putative phospholipid transport factor YdbH
RA 1,450,650 C→T E234K (GAA→AAA)  tynA ← copper‑containing amine oxidase
RA 1,452,971 G→A S224S (AGC→AGT paaZ ← fused 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase and oxepin‑CoA hydrolase
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,641,739 C→T intergenic (‑185/‑116) cspB ← / → cspF cold shock‑like protein CspB/cold shock‑like protein CspF
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,657,359 G→A F33F (TTC→TTT ynfC ← PF06788 family lipoprotein YnfC
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,681,779 G→A R84R (CGC→CGT ydgC ← GlpM family protein
RA 1,686,490 G→A F33F (TTC→TTT fumC ← fumarase C
RA 1,687,239 G→A S380F (TCT→TTT)  fumA ← fumarase A
RA 1,713,725 G→A E319E (GAG→GAA dtpA → dipeptide/tripeptide:H(+) symporter DtpA
RA 1,714,173 G→A A469T (GCC→ACC)  dtpA → dipeptide/tripeptide:H(+) symporter DtpA
RA 1,715,687 G→A A68V (GCC→GTC)  pdxY ← pyridoxal kinase 2
RA 1,719,802 G→A intergenic (‑200/‑74) anmK ← / → slyB anhydro‑N‑acetylmuramic acid kinase/outer membrane lipoprotein SlyB
RA 1,734,709 G→A S92N (AGC→AAC)  mepH → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,755,770 G→A D25N (GAC→AAC)  pykF → pyruvate kinase 1
RA 1,760,546 G→A Q416* (CAA→TAA)  sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,766,803 G→A P628L (CCA→CTA)  ydiJ ← putative FAD‑linked oxidoreductase YdiJ
RA 1,774,603 G→A G272D (GGC→GAC)  ydiB → quinate/shikimate dehydrogenase
RA 1,781,673 G→A E93E (GAG→GAA ydiS → putative electron transfer flavoprotein‑quinone oxidoreductase YdiS
RA 1,783,814 G→A D280N (GAT→AAT)  fadK → short chain acyl‑CoA synthetase
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,814,782 G→A E306K (GAA→AAA)  katE → catalase HPII
RA 1,831,079 G→A P302S (CCA→TCA)  astC ← succinylornithine transaminase
RA 1,837,466 G→A W85* (TGG→TGA ynjC → putative ABC transporter membrane subunit YnjC
RA 1,849,728 G→A E298K (GAA→AAA)  sppA → protease IV, a signal peptide peptidase
RA 1,849,860 G→A E342K (GAA→AAA)  sppA → protease IV, a signal peptide peptidase
RA 1,860,094 G→A L46F (CTC→TTC)  ydjK ← putative transporter YdjK
RA 1,863,734 G→A V322I (GTT→ATT)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,869,994 G→A G347D (GGC→GAC)  yeaH → DUF444 domain‑containing protein YeaH
RA 1,872,813 G→A W258* (TGG→TAG)  dgcJ → diguanylate cyclase DgcJ
RA 1,873,439 G→A A467T (GCG→ACG)  dgcJ → diguanylate cyclase DgcJ
RA 1,873,865 G→A E98K (GAA→AAA)  yeaK → mischarged aminoacyl‑tRNA deacylase
RA 1,889,610 C→T G46E (GGG→GAG)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,894,170 G→A G13D (GGT→GAT)  yoaC → DUF1889 domain‑containing protein YoaC
RA 1,956,470 C→T A180T (GCA→ACA)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 1,984,291 C→T R260H (CGC→CAC)  araG ← arabinose ABC transporter ATP binding subunit
RA 1,990,161 C→T L161L (CTG→TTG)  tyrP → tyrosine:H(+) symporter
RA 1,994,050 C→T Q219Q (CAG→CAA uvrC ← UvrABC excision nuclease subunit C
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,009,059 C→T I261I (ATC→ATT yedE → putative selenium transporter YedE
RA 2,019,834 C→T L73F (CTT→TTT)  fliL → flagellar protein FliL
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,035,891 C→T F19F (TTC→TTT hchA → protein/nucleic acid deglycase 1
RA 2,126,536 C→T A219T (GCG→ACG)  fcl ← GDP‑L‑fucose synthase
RA 2,159,473 C→T A363V (GCC→GTC)  mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,166,682 C→T L339L (CTG→TTG)  trhP → tRNA wobble base hydroxylation protein TrhP
RA 2,170,027 C→T pseudogene (141/475 nt) gatR ← DNA‑binding transcriptional repressor GatR, N‑terminal fragment
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,176,651 C→T S185N (AGC→AAC)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2
RA 2,176,721 C→T E162K (GAA→AAA)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2
RA 2,177,055 C→T T50T (ACG→ACA gatY ← tagatose‑1,6‑bisphosphate aldolase 2
RA 2,199,572 C→T intergenic (+2274/‑707) yehF → / → yehI PF05406 family protein YehF/DUF4132 domain‑containing protein YehI
RA 2,205,728 C→T H12Y (CAT→TAT)  yehM → uncharacterized protein YehM
RA 2,206,946 C→T P418S (CCT→TCT)  yehM → uncharacterized protein YehM
RA 2,208,284 C→T D106D (GAC→GAT yehP → VWA domain‑containing protein YehP
RA 2,210,242 C→T N381N (AAC→AAT yehQ → SWIM zinc finger domains‑containing protein YehQ
RA 2,215,552 C→T T229T (ACC→ACT mlrA → DNA‑binding transcriptional activator MlrA
RA 2,218,986 C→T A166T (GCA→ACA)  osmF ← glycine betaine ABC transporter periplasmic binding protein OsmF
RA 2,227,544 C→T intergenic (‑274/‑1438) yohF ← / → yohP putative oxidoreductase YohF/putative membrane protein YohP
RA 2,228,005 C→T intergenic (‑735/‑977) yohF ← / → yohP putative oxidoreductase YohF/putative membrane protein YohP
RA 2,233,751 C→T P51L (CCC→CTC)  yeiS → DUF2542 domain‑containing protein YeiS
RA 2,234,001 C→T intergenic (+162/‑32) yeiS → / → preT DUF2542 domain‑containing protein YeiS/dihydropyrimidine dehydrogenase (NAD(+)) subunit PreT
RA 2,240,166 C→T M61I (ATG→ATA mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,247,968 C→T G189S (GGC→AGC)  lysP ← lysine:H(+) symporter
RA 2,253,175 C→T G324E (GGA→GAA)  nupX ← putative nucleoside transporter
RA 2,253,186 C→T G320G (GGG→GGA nupX ← putative nucleoside transporter
RA 2,253,230 C→T A306T (GCG→ACG)  nupX ← putative nucleoside transporter
RA 2,254,400 C→T D263N (GAC→AAC)  rihB ← pyrimidine‑specific ribonucleoside hydrolase RihB
RA 2,255,699 C→T L115L (CTC→CTT yeiL → DNA‑binding transcriptional activator YeiL
RA 2,256,424 C→T P304P (CCG→CCA psuT ← putative pseudouridine transporter
RA 2,257,367 C→T intergenic (‑32/+62) psuT ← / ← psuG putative pseudouridine transporter/pseudouridine‑5'‑phosphate glycosidase
RA 2,259,431 C→T intergenic (‑135/+288) psuK ← / ← fruA putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,260,379 C→T E344E (GAG→GAA fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,260,455 C→T G319D (GGT→GAT)  fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,260,796 C→T P205P (CCG→CCA fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,291,308 G→A intergenic (+161/+50) narP → / ← ccmH DNA‑binding transcriptional dual regulator NarP/holocytochrome c synthase CcmH component
RA 2,292,586 G→A L127L (CTG→TTG)  ccmG ← thiol:disulfide oxidoreductase CcmG
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,320,632 C→T T197I (ACC→ATC)  atoS → sensor histidine kinase AtoS
RA 2,331,733 G→A intergenic (‑1936/+2603) yfaQ ← / ← yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
RA 2,332,012 G→A intergenic (‑2215/+2324) yfaQ ← / ← yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
RA 2,335,259 G→A D462D (GAC→GAT yfaA ← DUF2138 domain‑containing protein YfaA
RA 2,338,695 G→A I242I (ATC→ATT gyrA ← DNA gyrase subunit A
RA 2,340,254 G→A D230N (GAT→AAT)  ubiG → bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,565,649 G→A D412D (GAC→GAT eutA ← ethanolamine ammonia‑lyase reactivase EutA
RA 2,829,251 C→T H69Y (CAT→TAT)  srlR → DNA‑binding transcriptional repressor SrlR
RA 3,007,245 G→A M328I (ATG→ATA ygeW → putative carbamoyltransferase YgeW
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,385,165 G→A E155K (GAG→AAG)  argR → DNA‑binding transcriptional dual regulator ArgR
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,828 C→T P13L (CCC→CTC)  yhfK → putative transporter YhfK
RA 3,486,835 C→T I15I (ATC→ATT yhfK → putative transporter YhfK
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,647,011 C→T T238I (ACC→ATC)  gor → glutathione reductase (NADPH)
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,750,191 C→T R36W (CGG→TGG)  sgbE → L‑ribulose‑5‑phosphate 4‑epimerase SgbE
RA 3,823,908 C→T I503I (ATC→ATT spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,824,748 C→T T78T (ACC→ACT trmH → tRNA (Gm18) 2'‑O‑methyltransferase
RA 3,837,511 C→T L187L (CTG→TTG)  setC → putative arabinose exporter
RA 3,841,368 C→T G124G (GGG→GGA nepI ← purine ribonucleoside exporter
RA 3,843,551 C→T G80E (GGG→GAG)  adeQ ← adenine transporter
RA 3,844,769 C→T P269L (CCG→CTG)  adeD → adenine deaminase
RA 3,850,898 C→T M65I (ATG→ATA ilvN ← acetohydroxy acid synthase I subunit IlvN
RA 3,856,378 C→T intergenic (‑71/‑37) yidH ← / → yidI DUF202 domain‑containing inner membrane protein YidH/putative inner membrane protein YidI
RA 3,861,253 C→T intergenic (+77/+734) yidL → / ← glvB putative DNA‑binding transcriptional regulator YidL/putative PTS enzyme II component GlvB
RA 3,867,345 G→A N26N (AAC→AAT ibpA ← small heat shock protein IbpA
RA 3,870,698 C→T A345T (GCG→ACG)  dgoT ← D‑galactonate:H(+) symporter
RA 3,876,172 C→T A261T (GCG→ACG)  yidA ← sugar phosphatase YidA
RA 3,880,897 C→T A109T (GCG→ACG)  recF ← recombination mediator protein RecF
RA 3,885,855 C→T F260F (TTC→TTT yidC → membrane protein insertase YidC
RA 3,887,376 C→T F183F (TTC→TTT mnmE → 5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,919,455 C→T G135S (GGC→AGC)  atpA ← ATP synthase F1 complex subunit alpha
RA 3,929,123 C→T E161K (GAA→AAA)  viaA ← protein ViaA
RA 3,937,092 C→T T272I (ACC→ATC) 
noncoding (48/234 nt)
rbsB →
rbsZ →
ribose ABC transporter periplasmic binding protein
small regulatory RNA RbsZ
RA 3,953,258 G→A A259T (GCG→ACG)  ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,956,510 G→A E394K (GAA→AAA)  ilvA → threonine deaminase
RA 3,957,435 G→A A129V (GCG→GTG)  ilvY ← DNA‑binding transcriptional dual regulator IlvY
RA 3,958,012 G→A A15T (GCA→ACA)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,961,182 G→A G169E (GGG→GAG)  rep → ATP‑dependent DNA helicase Rep
RA 3,971,449 G→A G64S (GGC→AGC)  wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
RA 3,975,714 G→A G190D (GGC→GAC)  wecE → dTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase
RA 3,979,532 G→A S311N (AGT→AAT)  wzyE → enterobacterial common antigen polymerase
RA 3,979,634 G→A G345D (GGC→GAC)  wzyE → enterobacterial common antigen polymerase
RA 4,003,017 C→T M59I (ATG→ATA yigG ← inner membrane protein
RA 4,050,689 C→T G26E (GGA→GAA)  yihA ← GTP‑binding protein YihA
RA 4,052,851 C→T I269I (ATC→ATT hemN → coproporphyrinogen III dehydrogenase
RA 4,072,023 G→A A210V (GCT→GTT)  yihT ← 6‑deoxy‑6‑sulfofructose‑1‑phosphate aldolase
RA 4,076,209 C→T L22L (CTA→TTA)  yihY → PF03631 family membrane protein YihY
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,152,036 G→A P355S (CCG→TCG)  ppc ← phosphoenolpyruvate carboxylase
RA 4,182,549 C→T I435I (ATC→ATT rpoB → RNA polymerase subunit beta
RA 4,189,936 C→T L51F (CTT→TTT)  yjaZ → protein YjaZ
RA 4,195,326 C→T W258* (TGG→TGA thiC ← phosphomethylpyrimidine synthase
RA 4,345,258 C→T D101N (GAC→AAC)  yjdF ← DUF2238 domain‑containing inner membrane protein YjdF
RA 4,410,960 G→A E136E (GAG→GAA yjfJ → PspA family protein YjfJ
RA 4,411,873 C→T S191F (TCT→TTT)  yjfK → DUF2491 domain‑containing protein YjfK
RA 4,512,917 C→T G165S (GGC→AGC)  fecC ← ferric citrate ABC transporter membrane subunit FecC
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
MC JC 4,549,192 Δ39 bp coding (385‑423/903 nt) fimH → type 1 fimbriae D‑mannose specific adhesin

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 113753 113876 124 2 [1] [1] 2 guaC GMP reductase
* * ÷ NC_000913 164531 164632 102 2 [1] [1] 2 [hrpB] [hrpB]
* * ÷ NC_000913 177214 177315 102 3 [1] [1] 2 yadS PF03458 family protein YadS
* * ÷ NC_000913 221397 221501 105 2 [1] [1] 2 metI L‑methionine/D‑methionine ABC transporter membrane subunit
* * ÷ NC_000913 238236 238337 102 2 [1] [1] 2 [ykfM] [ykfM]
* * ÷ NC_000913 289251 289313 63 2 [1] [1] 2 [argF] [argF]
* * ÷ NC_000913 330932 331059 128 2 [1] [1] 2 betT choline:H(+) symporter
* * ÷ NC_000913 388691 388704 14 2 [1] [1] 2 tauD/hemB taurine dioxygenase/porphobilinogen synthase
* * ÷ NC_000913 406523 406713 191 2 [1] [1] 2 aroL shikimate kinase 2
* * ÷ NC_000913 421904 421993 90 2 [1] [1] 2 proY putative transporter ProY
* * ÷ NC_000913 442192 442202 11 2 [0] [1] 2 thiI tRNA uridine 4‑sulfurtransferase
* * ÷ NC_000913 459112 459170 59 4 [1] [0] 2 lon Lon protease
* * ÷ NC_000913 488196 488239 44 2 [0] [1] 2 mscK potassium dependent, small conductance mechanosensitive channel
* * ÷ NC_000913 497782 497844 63 2 [1] [1] 2 [adk] [adk]
* * ÷ NC_000913 628788 628822 35 3 [1] [1] 2 entA 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
* * ÷ NC_000913 630491 630569 79 2 [1] [1] 2 cstA pyruvate transporter CstA
* * ÷ NC_000913 641180 641376 197 2 [1] [1] 2 [ahpF] [ahpF]
* * ÷ NC_000913 645217 645363 147 2 [1] [1] 2 citT citrate:succinate antiporter
* * ÷ NC_000913 753712 753717 6 2 [1] [0] 2 gltA citrate synthase
* * ÷ NC_000913 766668 766744 77 2 [1] [1] 2 mngA 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
* * ÷ NC_000913 807277 807363 87 3 [1] [1] 2 [ybhC] [ybhC]
* * ÷ NC_000913 814833 814841 9 2 [1] [1] 2 uvrB UvrABC excision nuclease subunit B
* * ÷ NC_000913 844951 845137 187 2 [1] [1] 2 ybiO moderate conductance mechanosensitive channel YbiO
* * ÷ NC_000913 847826 847968 143 2 [1] [1] 2 glnH L‑glutamine ABC transporter periplasmic binding protein
* * ÷ NC_000913 858486 858628 143 2 [1] [1] 2 ybiU DUF1479 domain‑containing protein YbiU
* * ÷ NC_000913 895198 895326 129 2 [1] [1] 2 potG putrescine ABC transporter ATP binding subunit
* * ÷ NC_000913 907522 907550 29 2 [1] [1] 2 ybjT putative NAD(P)‑binding protein YbjT
* * ÷ NC_000913 913108 913198 91 2 [1] [1] 2 hcp protein S‑nitrosylase
* * ÷ NC_000913 987419 987503 85 2 [1] [1] 2 ompF/asnS outer membrane porin F/asparagine‑‑tRNA ligase
* * ÷ NC_000913 994346 994510 165 2 [1] [1] 2 ssuC aliphatic sulfonate ABC transporter membrane subunit
* * ÷ NC_000913 1005113 1005152 40 3 [1] [1] 2 pyrD dihydroorotate dehydrogenase, type 2
* * ÷ NC_000913 1012386 1012416 31 3 [1] [1] 2 pqiA intermembrane transport protein PqiA
* * ÷ NC_000913 1065176 1065234 59 2 [1] [1] 2 yccE uncharacterized protein YccE
* * ÷ NC_000913 1168776 1168833 58 2 [0] [1] 2 [comR] [comR]
* * ÷ NC_000913 1186967 1187063 97 2 [1] [1] 2 pepT peptidase T
* * ÷ NC_000913 1192460 1192473 14 2 [1] [1] 2 hflD lysogenization regulator
* * ÷ NC_000913 1196245 1211513 15269 2 [1] [0] 2 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1211852 1211885 34 2 [0] [1] 2 iraM anti‑adaptor protein IraM
* * ÷ NC_000913 1231687 1231806 120 2 [1] [1] 3 umuC DNA polymerase V catalytic protein
* * ÷ NC_000913 1275694 1275824 131 2 [1] [1] 2 narL DNA‑binding transcriptional dual regulator NarL
* * ÷ NC_000913 1299388 1300869 1482 2 [1] [1] 3 insH21 insH21
* * ÷ NC_000913 1313636 1313711 76 2 [1] [1] 2 [yciC] [yciC]
* * ÷ NC_000913 1331514 1331586 73 2 [1] [1] 3 topA DNA topoisomerase 1
* * ÷ NC_000913 1346211 1346260 50 2 [0] [1] 2 pdeR cyclic di‑GMP phosphodiesterase PdeR
* * ÷ NC_000913 1349709 1349888 180 2 [1] [1] 2 yciW putative peroxidase YciW
* * ÷ NC_000913 1351195 1351365 171 2 [1] [1] 2 fabI/ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
* * ÷ NC_000913 1355696 1355766 71 2 [1] [1] 4 sapA putative periplasmic binding protein SapA
* * ÷ NC_000913 1359707 1359757 51 2 [1] [0] 2 puuA glutamate‑putrescine ligase
* * ÷ NC_000913 1359813 1359818 6 2 [1] [0] 2 puuA glutamate‑putrescine ligase
* * ÷ NC_000913 1416978 1417113 136 2 [1] [1] 2 recE exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
* * ÷ NC_000913 1420028 1420038 11 2 [0] [1] 2 racR DNA‑binding transcriptional repressor RacR
* * ÷ NC_000913 1485770 1485845 76 2 [1] [1] 2 hrpA ATP‑dependent 3'‑>5' RNA helicase HrpA
* * ÷ NC_000913 1518023 1518149 127 2 [1] [1] 2 ydcZ putative inner membrane protein YdcZ
* * ÷ NC_000913 1519911 1520054 144 2 [1] [1] 2 curA NADPH‑dependent curcumin/dihydrocurcumin reductase
* * ÷ NC_000913 1581792 1581941 150 2 [1] [1] 2 ydeN putative sulfatase YdeN
* * ÷ NC_000913 1598766 1598794 29 2 [1] [0] 2 lsrK autoinducer‑2 kinase
* * ÷ NC_000913 1608719 1608723 5 2 [0] [1] 2 yneE PF01062 family inner membrane protein YneE
* * ÷ NC_000913 1626287 1626445 159 2 [1] [1] 3 dcp peptidyl‑dipeptidase Dcp
* * ÷ NC_000913 1650218 1650372 155 2 [1] [1] 2 ydfD/ynfP lysis protein/protein YnfP
* * ÷ NC_000913 1715224 1715323 100 2 [1] [1] 3 pdxY pyridoxal kinase 2
* * ÷ NC_000913 1720972 1721039 68 2 [1] [1] 3 [ydhI] [ydhI]
* * ÷ NC_000913 1749602 1749669 68 2 [1] [1] 2 ydhU putative cytochrome YdhU
* * ÷ NC_000913 1823861 1823948 88 2 [1] [1] 2 cho excinuclease Cho
* * ÷ NC_000913 1844858 1844931 74 3 [1] [1] 2 [ynjI] [ynjI]
* * ÷ NC_000913 1941069 1941187 119 2 [1] [1] 2 mepM peptidoglycan endopeptidase MepM
* * ÷ NC_000913 1961968 1961999 32 2 [1] [1] 3 [yecT] [yecT]
* * ÷ NC_000913 1963833 1963903 71 2 [1] [1] 3 flhA flagellar biosynthesis protein FlhA
* * ÷ NC_000913 1978495 1979270 776 11 [0] [0] 5 insB5–insA5 insB5,insA5
* * ÷ NC_000913 1995522 1995540 19 2 [1] [1] 2 uvrY/yecU DNA‑binding transcriptional activator UvrY/protein YecU
* * ÷ NC_000913 2005352 2005393 42 2 [1] [1] 2 fliS flagellar biosynthesis protein FliS
* * ÷ NC_000913 2100805 2100865 61 2 [1] [1] 2 gnd 6‑phosphogluconate dehydrogenase, decarboxylating
* * ÷ NC_000913 2109370 2109416 47 3 [1] [0] 3 rfbX polyisoprenol‑linked O16‑antigen repeat unit flippase
* * ÷ NC_000913 2184652 2184757 106 2 [1] [0] 2 thiM hydroxyethylthiazole kinase
* * ÷ NC_000913 2245179 2245231 53 2 [1] [1] 2 cirA iron‑catecholate outer membrane transporter CirA
* * ÷ NC_000913 2280899 2281105 207 2 [1] [1] 2 radD putative DNA repair helicase RadD
* * ÷ NC_000913 2305365 2305391 27 2 [1] [1] 2 mqo malate:quinone oxidoreductase
* * ÷ NC_000913 2328057 2328124 68 2 [1] [1] 2 yfaP DUF2135 domain‑containing protein YfaP
* * ÷ NC_000913 2332518 2332661 144 2 [1] [1] 2 yfaQ/yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
* * ÷ NC_000913 2383936 2383988 53 2 [1] [1] 2 elaD/yfbK protease ElaD/IPR002035/DUF3520 domain‑containing protein YfbK
* * ÷ NC_000913 2396662 2396724 63 2 [1] [1] 2 nuoH NADH:quinone oxidoreductase subunit H
* * ÷ NC_000913 2404882 2404956 75 2 [1] [1] 2 nuoA NADH:quinone oxidoreductase subunit A
* * ÷ NC_000913 2411887 2411930 44 2 [1] [1] 2 hxpA hexitol phosphatase A
* * ÷ NC_000913 2419428 2419630 203 2 [1] [1] 2 yfcE phosphodiesterase YfcE
* * ÷ NC_000913 2437983 2438121 139 2 [1] [1] 2 flk putative flagella assembly protein
* * ÷ NC_000913 2469345 2469467 123 2 [1] [1] 2 yfdI serotype‑specific glucosyl transferase YfdI
* * ÷ NC_000913 2506805 2506836 32 2 [1] [1] 2 fryC putative PTS enzyme IIC component FryC
* * ÷ NC_000913 2594741 2594789 49 2 [1] [1] 2 tmcA tRNA(Met) cytidine acetyltransferase
* * ÷ NC_000913 2636154 2636164 11 2 [1] [0] 2 der 50S ribosomal subunit stability factor
* * ÷ NC_000913 2682101 2682173 73 2 [1] [1] 2 yphG DUF5107 domain‑containing protein YphG
* * ÷ NC_000913 2905607 2905676 70 2 [1] [1] 2 yqcG cell envelope stress response protein YqcG
* * ÷ NC_000913 2966381 2966493 113 2 [1] [1] 2 ptsP phosphoenolpyruvate‑protein phosphotransferase PtsP
* * ÷ NC_000913 3019481 3019539 59 2 [1] [1] 2 ssnA putative aminohydrolase SsnA
* * ÷ NC_000913 3035413 3035477 65 2 [1] [1] 2 prfB peptide chain release factor RF2
* * ÷ NC_000913 3045982 3046060 79 2 [1] [1] 2 ygfF/gcvP putative oxidoreductase YgfF/glycine decarboxylase
* * ÷ NC_000913 3239505 3239707 203 2 [1] [1] 2 [alx] [alx]
* * ÷ NC_000913 3364895 3364919 25 2 [1] [1] 2 yhcD putative fimbrial usher protein YhcD
* * ÷ NC_000913 3406845 3406868 24 2 [1] [1] 2 accC biotin carboxylase
* * ÷ NC_000913 3417149 3417277 129 4 [1] [1] 3 acrF multidrug efflux pump RND permease AcrF
* * ÷ NC_000913 3423825–3424233 3424468–3424240 8–644 2 [1] [1] 2 [rrlD] [rrlD]
* * ÷ NC_000913 3458892 3458935 44 2 [0] [1] 2 gspE Type II secretion system protein GspE
* * ÷ NC_000913 3504885 3504905 21 3 [1] [1] 2 frlR/yhfS DNA‑binding transcriptional regulator FrlR/putative aminotransferase YhfS
* * ÷ NC_000913 3608405 3608434 30 2 [0] [1] 2 zntA Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
* * ÷ NC_000913 3781022 3781151 130 2 [1] [1] 2 lldD/trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
* * ÷ NC_000913 3797146 3797162 17 2 [1] [1] 2 waaL O‑antigen ligase
* * ÷ NC_000913 3926307 3926414 108 2 [1] [1] 2 mioC flavoprotein MioC
* * ÷ NC_000913 3959932 3959948 17 2 [0] [1] 2 ppiC/rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
* * ÷ NC_000913 3986441 3986461 21 2 [1] [0] 2 glmZ small regulatory RNA GlmZ
* * ÷ NC_000913 4065992 4066071 80 2 [0] [1] 2 yihP putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
* * ÷ NC_000913 4070336 4070531 196 2 [1] [1] 2 [yihR]–[yihS] [yihR],[yihS]
* * ÷ NC_000913 4174025 4174142 118 2 [1] [1] 2 [birA]–[coaA] [birA],[coaA]
* * ÷ NC_000913 4224828 4224925 98 2 [1] [1] 2 metH cobalamin‑dependent methionine synthase
* * ÷ NC_000913 4248150 4248206 57 2 [1] [1] 2 lamB maltose outer membrane channel/phage lambda receptor protein
* * ÷ NC_000913 4494614 4494679 66 2 [1] [1] 2 [idnK] [idnK]
* * ÷ NC_000913 4549834 4549934 101 2 [1] [1] 2 fimH/gntP type 1 fimbriae D‑mannose specific adhesin/fructuronate transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 279163 =NA (NA)11 (1.340) 8/334 0.1 91.7% noncoding (768/768 nt) IS1 repeat region
?NC_000913 2666074 = 1 (0.120)coding (640/1281 nt) csiE stationary phase‑inducible protein CsiE
* ? NC_000913 = 1397238NA (NA)11 (1.340) 10/334 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)5 (0.610) 4/334 0.4 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)12 (1.470) 9/332 0.0 92.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 4536236 = 1 (0.120)intergenic (‑2183/‑361) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
* ? NC_000913 4135180 =3 (0.370)11 (1.340) 6/334 0.2 78.6% coding (1346/2181 nt) katG catalase/hydroperoxidase HPI
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region