Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,835	C→T	I15I (ATC→ATT)	yhfK →	putative transporter YhfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,835	0	C	T	100.0%	25.4 / NA	11	I15I (ATC→ATT)	yhfK	putative transporter YhfK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

CGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACT  >  NC_000913/3486605‑3487041                                                                                                                                                                                                                                       |                                                                                                                                                                                                               cGGTATGCACATCAACATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTACAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCTCGATATTAACTATGCACTTCGACaaa                                                                                                                                                                                             >  1:136899/1‑250 (MQ=255)                                           tGTCGGCTGTTCTCGTGAAACCGGGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGGCTGAGTGGCGCGTGTCCTGGTAGCGCGCCATATTGTTTCCCCCGATGTGGCGCAGACTGATTTATCTCCTCGATATTAACTATGCACTTCGACAAACGTTGGTGCTATGTTTGCCCGTGGCCGTTTGGTTAATGCTTg                                                                                                                                                   <  1:116359/250‑1 (MQ=255)                                            gTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTTATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCCTTTTGTTTCCCCCGAGGTGGCGCAGACTGATTTATCACCTCGATATTAAATATGCACTTCGAAAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAAAGCTTGGc                                                                                                                                                 >  1:22798/1‑251 (MQ=255)                                            gTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCTCGATATTAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCg                                                                                                                                                                        >  1:133742/1‑228 (MQ=255)                                            gTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCTCGATATTAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTggn                                                                                                                                                 <  1:135414/251‑2 (MQ=255)                                              cGGCTGTTCTCGTGAAACCGTGGGACNCATTCTNNAGNTGCTGGAAGATCAGAACCANATNNNCNNNCNAGGTANAANNCTCGTCGTTNACGGCACTCGTTACTCCCGTCGGAGTGGCGCGTTACCTGGTCGCGCGTCCTTTNGTTTCCACCNNTGTGGCGCAGACTGATTTATCNCCTNNANNTNAAcnttn                                                                                                                                                                                                         >  2:34979/1‑189 (MQ=255)                                              cGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGTCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTCTCCCCTCGATATTAACTATGTAATTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCTTTTGGTTAATGc                                                                                                                                                      <  1:34979/244‑1 (MQ=255)                                                     tctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATTTGGCGCAGACTGATTTATCACCTCGATATTAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTaan                                                                                                                                                        <  1:48941/235‑2 (MQ=255)                                                                               gAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTGTCACCTCGATATTAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCTTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTc                                                                                                               <  1:162708/250‑1 (MQ=255)                                                                                       tGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATACCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCTCGATATTAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGtt                                                                                                      >  1:70840/1‑251 (MQ=255)                                                                                                                                                                    gcgTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCTCGATATTCACTATGAACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCAcc                         >  1:27822/1‑251 (MQ=255)                                                                                                                                                                                            tgtttctaccgctgtcgcgcCGACTGATTTAATACCCCCCTATTAACTATGCACTTCGACAAACGCAGATGCTACGTTTGCCAGAGGCAGTTGGGTTAAAGCCTGGCGAATTACGATTCGGTCTGCCATTTTCCCCCGATCCTGCCAGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGGTTTTTCAAACGCTTATTCCTTGGGGCGTCGCTGTTTGCCACCTGTAGCTTTCTGACACAGctact  >  1:142027/17‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                               CGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGTTTGCCACCTGTAGCTTGCTGACACAGCTACT  >  NC_000913/3486605‑3487041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑