Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,841,368	C→T	G124G (GGG→GGA)	nepI ←	purine ribonucleoside exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,841,368	0	C	T	100.0%	16.7 / NA	7	G124G (GGG→GGA)	nepI	purine ribonucleoside exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

CTGGCAGTGAAGGCAATGATTTGATAATCCAGAAAATACACAGCACGCCCATCACCGCCGCCGCATTAAAGACATTGCGCCAACCGATAAGCTCGCCTAAAAAACTGCCCAACGGCGCGGCAATCACCAGCGCAATAGAAACCGCGCCGAAGATCACCGACAGCGCCTTCGGCACCGTACGCGGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAACCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGAGAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGC  >  NC_000913/3841142‑3841538                                                                                                                                                                                                                                   |                                                                                                                                                                           cTGGCAGTGAAGGCAATGATTTGATAATCCAGAAAATACACAGCACGCCCATCCCAGCCGCCGCATTACCGACATTGCGCCAACCGATAAGCTCGCCTAAAAAACTGCCCAACGGCGCGGCAATCCCCACCGCAATAGAAACCGCGCCGAAGATCACCGACAGCGCCTTCGGCACCGTACGCGGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACa                                                                                                                                                     >  1:94701/1‑250 (MQ=255)                                                                             gcgcCAACCGATAAGCTCGCCTAAAAAACTGCCCAACGGCGCGGCAATCACCAGCGCAATAGAAACCGCGCCGAAGATCACCGACAGCGCCTTCGGCACCGTACGCGGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGAGAGCGTCAGCAAAACGGca                                                                         >  1:67326/1‑250 (MQ=255)                                                                                                                                                   cnGAAGATCACCGACAGCGCCTTCGGCACCGTACGCCGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGCGAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGGAATAAACAAACTGGCAAACATTGc  >  1:131410/1‑251 (MQ=255)                                                                                                                                                     gAAGATCACCGACAGCGCCTTCGGCACCGTACGCGGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAgg                                                                                             <  1:132741/158‑1 (MQ=255)                                                                                                                                                                                         cggcACCAGACGCATGGTCAGCGACGCCGACATCGCCAAGAATCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCGGGAGAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTgg            >  1:97479/1‑203 (MQ=255)                                                                                                                                                                                                ngACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACAGGAACGACCGATTAAAAGAAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGAGAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCCGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACaatg   >  1:16694/2‑202 (MQ=255)                                                                                                                                                                                                 gACGCATGGTCAGCGACGCCGACATCGCCCAGAATCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGagag                                                                                         >  1:70278/1‑118 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                           CTGGCAGTGAAGGCAATGATTTGATAATCCAGAAAATACACAGCACGCCCATCACCGCCGCCGCATTAAAGACATTGCGCCAACCGATAAGCTCGCCTAAAAAACTGCCCAACGGCGCGGCAATCACCAGCGCAATAGAAACCGCGCCGAAGATCACCGACAGCGCCTTCGGCACCGTACGCGGCGGCACCAGACGCATGGTCAGCGACGCCGACATCGCCCAGAACCCGCCCAGCGCCAGCCCCAGACAGGCACGACCGATTAAAAGCAAACTGAATGAGTTAGCAAAGGAAACCAGCAAGCAGGAGAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGC  >  NC_000913/3841142‑3841538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑