Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,165	G→A	E155K (GAG→AAG)	argR →	DNA‑binding transcriptional dual regulator ArgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,165	0	G	A	100.0%	28.6 / NA	13	E155K (GAG→AAG)	argR	DNA‑binding transcriptional dual regulator ArgR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

AGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAATAGTGCGTTTTACTGCGACATGTCATTCACACAATGAATACATAAGGTAAAAAAAGCACATTATGCAAAATTCATTATCTAATTGAAAAAACTAGAATTAACGATAAATAACCGTATTTTTAATTCTTTT  >  NC_000913/3384950‑3385388                                                                                                                                                                                                                        |                                                                                                                                                                                                                                agaaTCTGGTGCTGGATATAGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGc                                                                                                                                                                                                                              >  1:66474/1‑219 (MQ=255)                                                            ggcgcggcgcAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAAt                                                                                                                                   <  1:158097/251‑1 (MQ=255)                                                                                                cTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGAACAGAAGCTTTAATCTCTACCCCGTCGTTTATGACGGCGGGGAAAATGTTGCTTATCCCTCTCACCCCCCTGCTTTCCCCTGCGATTAAATTAACGAATAGTGTGTTTTACTGCGACATGTCATTTACACAATg                                                                                                >  1:117692/1‑250 (MQ=255)                                                                                                         ncAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCCGCGATTAATTTAACGAATAGTGCGTTTTTCTGCGAAATGTAATTccca                                                                                                     >  1:99699/2‑233 (MQ=255)                                                                                                                          cACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTTTCCCTCTCAACCCCCTGCTTTCCCCTGCGCTTAATTTTAAGAATAGTGCGTTTTACTGCGACATGTCATTCACACCATGAATACATAAGGGAAAAAAAGCACAtt                                                                      >  1:114274/1‑250 (MQ=255)                                                                                                                            ccATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCCGAAGCTTTAATCTCTGCCCcgtcg                                                                                                                                                                                                           >  1:147795/1‑115 (MQ=255)                                                                                                                                     gCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCggg                                                                                                                                                                                             >  1:126227/1‑120 (MQ=255)                                                                                                                                     gCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAATAGTGCNt                                                                                                                            <  1:132212/185‑1 (MQ=255)                                                                                                                                     gCGATGACACCATCTTTACCACCCCNGCTAACGGTTNCACNNTCAANGNCNTGTACGAAGCGATTTTAGAGCTGTTCGACNAGAAGCTTTAATCTCTGCCCCGTNNTTNNNGANNGCggg                                                                                                                                                                                             <  2:126227/120‑1 (MQ=255)                                                                                                                                                            ccNGCTAANGGTTNCACAGTCAAAGACCTNTACGAAGCGATTTTAGAGCNNTNCGACCNGAAGc                                                                                                                                                                                                                              <  2:66474/64‑1 (MQ=255)                                                                                                                                                                                           aCGAAGCGATTTTAGAGCTGTTCGACCAGAAGCTTTACTCTCTGCCCCGTCGCTTCTGACGGGGGGGAAAACGTTGTTTCTTACTCTCAACCCCCCGCTTTCCCCAGCGATTACTTTAACGAATAGTGCGTTTTACTGCGACCTGTAAttta                                                                                                       >  1:145938/1‑150 (MQ=255)                                                                                                                                                                                             gAAGCGATTTTAGAGCTGTTCGACCAGAATCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAATAGTGCTTTTTACTGCGACATTTCATTCACACAATGAATACATAAGGTAAAAAAAGCACATTATGCAAAAATTATTATCTAATTGAAATAACTAGAATTAACGATAAATAACCGTATTTTTAATTCtttt  <  1:168474/251‑1 (MQ=255)                                                                                                                                                                                                              gNTCGACCAGAAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAATAGTGCGTTTTACTGCGACATGTCATTcaca                                                                                                     <  2:99699/135‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                AGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAGCTTTAATCTCTGCCCCGTCGTTTCTGACGGCGGGGAAAATGTTGCTTATCCCTCTCAACCCCCTGCTTTCCCCTGCGATTAATTTAACGAATAGTGCGTTTTACTGCGACATGTCATTCACACAATGAATACATAAGGTAAAAAAAGCACATTATGCAAAATTCATTATCTAATTGAAAAAACTAGAATTAACGATAAATAACCGTATTTTTAATTCTTTT  >  NC_000913/3384950‑3385388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑