Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,750,191	C→T	R36W (CGG→TGG)	sgbE →	L‑ribulose‑5‑phosphate 4‑epimerase SgbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,750,191	0	C	T	100.0%	21.1 / NA	10	R36W (CGG→TGG)	sgbE	L‑ribulose‑5‑phosphate 4‑epimerase SgbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/8);  total (2/8)

GAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGACCGCCGACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGATACACCAACGCATCTGGCGCTCTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATACCCACTCG  >  NC_000913/3749969‑3750379                                                                                                                                                                                                                               |                                                                                                                                                                                             gAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTTGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCg                                                                                                                                                                   <  1:137021/250‑1 (MQ=255)                      gATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGAc                                                                                                                                             <  1:55795/251‑1 (MQ=255)                          gagaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGGTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGGAATCAAACCGTCCGGCGTCGAGTTCGACGtgattcccgc                                                                                                                                          <  1:47529/250‑7 (MQ=255)                                              cAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGGGTTTCCCGCTCTCCAGCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGACACTTGGCAATGGATGGTAAGCAAACCTGCCGGCGTCGAGTACGACGTGATGACCGCCGACGATATGGTGGTGGTTga                                                                                                                     <  1:65695/251‑1 (MQ=255)                                                                        aGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTGCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAAGGTCAGCGCTGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGGGATGACCGCCGACGAGATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAg                                                                                           <  1:156727/251‑1 (MQ=255)                                                                                               cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCGGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGGACGACGTGATGACCGCCGACGATATGGTGGTTGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGAt                                                                    <  1:149568/251‑1 (MQ=255)                                                                                                                            gagCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGACCGCCGACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGATACACCAACGCATCTGGCGCTCTACCGTCg                                       >  1:3049/1‑251 (MQ=255)                                                                                                                                                                 cgcTTCCCGCTCACCATCTGGTGACGGTCTCCTGGGGCAATGTCAGCGCGGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGGCGTGATGACCGCCGACGATATGGGGGTGGTTGAGATAGCCAGCTGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGATACTCCAACGCATCTGGCGCTCTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATACCCATTcg  <  1:173583/251‑1 (MQ=255)                                                                                                                                                                                                               cgcgGTAGACGAAACGTGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGCCCGCCGACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGAAAAAAACCCTCTTCCGATACACCAACGCATCTGGCGCTCTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATAcn        <  1:99941/199‑2 (MQ=255)                                                                                                                                                                                                               cgcgGCAGACGAGACGTGACAATGGATGGTAATCAAACCTTTCGGCGTCGAGTACGACGTGATGACCGCCGACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTNCCGNNNNNccnnnnnnnnnn                                                     >  2:99941/1‑144 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                             GAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGACCGCCGACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGATACACCAACGCATCTGGCGCTCTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATACCCACTCG  >  NC_000913/3749969‑3750379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑