Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	665,500	G→A	L272L (CTG→TTG)	mrdB ←	peptidoglycan glycosyltransferase MrdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,500	0	G	A	100.0%	17.9 / NA	9	L272L (CTG→TTG)	mrdB	peptidoglycan glycosyltransferase MrdB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

ACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACGCGCTGGCGCTGGTAATCATGCATCAGGAAGAACCACAGAATCG  >  NC_000913/665340‑665727                                                                                                                                                                 |                                                                                                                                                                                                                                    accataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAACGCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGc                                                                                                                                            <  1:126777/250‑1 (MQ=255)            atTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAACTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATccg                                                                                                                                 <  1:128955/250‑1 (MQ=255)            atTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAACTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATccg                                                                                                                                 <  1:128956/250‑1 (MQ=255)                           aaCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAACTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCa                                                                                                                  >  1:140019/1‑250 (MQ=255)                                   atatCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTGGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATTTAGAGAGCGAGCATAATCAGAATGCCCACTAATCCCAACTCTTCCGCCAGTACCGCTAAGATAAAGTCAGTATgg                                                                                                                                                                                                <  1:140764/164‑1 (MQ=255)                                                    ccagccaTGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGACTGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAACTCTTCCGCCAGTCCCGCGAAGATAAAGTCAGTCTGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGcc                                                                                        >  1:165903/1‑251 (MQ=255)                                                                          tggtTTGCGCTCTGGCGGCTATCCACAGCACGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAAATCTTCCGCCAGTACCGCGAAGATCAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTcc                                                                  >  1:51889/1‑251 (MQ=255)                                                                                                 cacaGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCAAACTAATCACAACTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTAcgcg                                           >  1:154909/1‑251 (MQ=255)                                                                                                                                          aatcagaatTCCCACTAATCCCAACTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCCTAATCCGCCGGAGCCAATAGCAATTTAAGACTGAATAATGTGATAGCCCGCTCCGAGTGTGTCTTATTCCGCGTCCAGTCGCATCATTACGCGCTGGCGCTGGTAATCATGCATCAGGAAGAACCACAGAATCg  <  1:146974/251‑1 (MQ=255)                                                                                                                                                                 |                                                                                                                                                                                                                                    ACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACGCGCTGGCGCTGGTAATCATGCATCAGGAAGAACCACAGAATCG  >  NC_000913/665340‑665727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑