Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,885,855	C→T	F260F (TTC→TTT)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,885,855	0	C	T	100.0%	24.4 / NA	11	F260F (TTC→TTT)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/9);  total (2/9)

CAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTC  >  NC_000913/3885616‑3886098                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    cAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATGCCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATGTTGCGACGGCGtn                                                                                                                                                                                                                                          <  1:135978/251‑2 (MQ=255)                                        ggTCAGGTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGGATGAGAAATACAAGTTCGAGACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATATTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCg                                                                                                                                                                                                    <  1:172336/250‑1 (MQ=255)                                                                                                  tCGCACTGCACACCTTCCGTGGCGCGNCGTACTNNACNCCTGACGAGAAGTATGAGANATNNNANNNCNCTACCNTTNNCGCTAACGANAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTNGCGACGGCGNNGATCCCACATAACGACGGTACNAACNNCNNCNATANCgn                                                                                                                                                                                                   >  2:34913/1‑192 (MQ=255)                                                                                                                 tCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTGCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTgg                                                                                                                           <  1:45/250‑1 (MQ=255)                                                                                                                      gtcgcgGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACGTCTATACCGCTAATCTGGGGAACGGCAGCGCCGCGATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGCCTGGCGCGAt                                                                                                                     <  1:161805/249‑1 (MQ=255)                                                                                                                        cgcgGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATg                                                                                                                    >  1:6218/1‑250 (MQ=255)                                                                                                                                actccactccTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTGCTATACCGCTAATCTGGGTAACGGCATCGCCGCTAGCTGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCAc                                                                                                            <  1:25175/250‑1 (MQ=255)                                                                                                                                 cTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATGCCGCTAATCTGGGTAACGTCATCGCCTCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTgg                                                                                                                           <  1:106679/234‑1 (MQ=255)                                                                                                                                                                                 cGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACGGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCgn                                                          <  1:20378/251‑2 (MQ=255)                                                                                                                                                                                  gATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTg                                                                                                                                                                                            >  1:22203/1‑120 (MQ=255)                                                                                                                                                                                   aTAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTTGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCAc                                                        <  1:37879/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGTATTTTGCGACGGCGTGGATCCCACATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTc  <  1:34913/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCGCTAATCTGGGTAACGGCATCGCCGCTATCGGCTATAAATCTCAGCCGGTACTGGTTCAGCCTGGTCAGACTGGCGCGATGAACAGCACCCTGTGGGTTGGCCCGGAAATCCAGGACAAAATGGCAGCTGTTGCTCCGCACCTGGATCTGACCGTTGATTACGGTTGGTTGTGGTTCATCTCTCAGCCGCTGTTC  >  NC_000913/3885616‑3886098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑