Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,001	C→T	intergenic (+162/‑32)	yeiS → / → preT	DUF2542 domain‑containing protein YeiS/dihydropyrimidine dehydrogenase (NAD(+)) subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,001	0	C	T	88.9%	11.1 / NA	9	intergenic (+162/‑32)	yeiS/preT	DUF2542 domain‑containing protein YeiS/dihydropyrimidine dehydrogenase (NAD(+)) subunit PreT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/4);  minor base G (0/1);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTC  >  NC_000913/2233813‑2234239                                                                                                                                                                                             |                                                                                                                                                                                                                                               aTTGTTTATCTTATTTTCTATCGTTAACATTCCCATCTCCAGTTAATTATAGAGCATAATTCTTACTTCAACTGATAAGCTGCGGCTCTCACCTACCCAACCGAAGCAATTTGTGAATCTTTTCACAGTTTAAATTCACCCGCACGCTTAGCCTTAATATCAGAACACTATTATTTACTCAACGCTCCTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGAAACAAAATAATATGGATGAactcc                                                                                                                                                                                  >  1:62804/1‑250 (MQ=255)       tATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTAGTGAGAATAATTAGTACTTCACCTGATAAGCTGCGTATATCACATTCCGAACCGCAGCTATGTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACGAACCGCTCGGCTTAATTCCCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTccn                                                                                                                                                                             <  1:29019/250‑2 (MQ=255)                          aaTATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGTAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTgac                                                                                                                                                         <  1:153370/251‑3 (MQ=255)                                             aaTTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTGACTAAACGCTCGTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCGTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                                                                      <  1:50167/251‑1 (MQ=255)                                                                 ccTTCACCTGATAAGTTGCTGAGATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGTTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGTCTTAATTACTTATAGCATGAAGGAAGATCACTTGCCGCAACAAAATTATCTGGATGAACTCTCTCCGGCTTTTACGTCTTTACTGGCTATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGc                                                                                                                  <  1:116762/250‑1 (MQ=255)                                                                                                                                                       nccTTAATATCAGTACATTATTATTTACTAAACGCTCGTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACACAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCtctc                                                                                                                                        >  1:32423/2‑143 (MQ=255)                                                                                                                                                       nccTTAATATCAGTACATTATTATTTACTAAACGCTCGTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCg                            >  1:103364/2‑251 (MQ=255)                                                                                                                                                        ccTTAATATCAGTCCATTATTATTTCCTAAACGCTCGTCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCCCTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCga                           <  1:3211/251‑1 (MQ=255)                                                                                                                                                                                 aCTAAACGCTCGTCTTAATTACCTATAGCCTTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGCAaatcggtggtgt  >  1:51490/1‑242 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                                               ATTGTTTATCTTATTTTCTATCGTTAATATTCCCCTCTCCAGTTAATTATTGAGAATAATTATTACTTCACCTGATAAGCTGCGGATATCACATTCCTAACCGCAGCTATTTGTGAATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTC  >  NC_000913/2233813‑2234239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑