Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	510,440	G→A	L314L (CTG→TTG)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	510,440	0	G	A	100.0%	11.4 / NA	7	L314L (CTG→TTG)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGG  >  NC_000913/510210‑510675                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ngCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTTCAACGCCTTAGAAGAACGCTGGc                                                                                                                                                                                                                         >  1:15758/2‑251 (MQ=255)      ggACGCTATCGCCTTCGCCTTTTTGCTGCTGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTCCCGGCCCGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGCCTGCACTTCTGCCAGAGGCACGCTTTTTTCTCCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAACGCCTTAGAAGAACGCTGGCGTgn                                                                                                                                                                                                                     <  1:42279/250‑2 (MQ=255)             aTCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTAAGCATCGCTTCAGCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGACACGCGAACGCCGGGCTTGATGCGCAGCAACATACCTGGCGGCACTTCTGCCAGAGGCACGCAGTTTTCACCGTCGTCAGTAACCAAGCGTGCCGTCGGCGGGTGTAAAGCGAGCAGCGTTTCCAACGCCTTAGAAGAACTCTGGCGTgcgcgcgc                                                                                                                                                                                                               <  1:167111/249‑1 (MQ=255)                               ctgcGGGATTGGTTCGCCCGTCCGCATAGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAACGCCTTAGAAGAACGCTGGCGAGCGCGAGCTTCCAGCATATGGCCg                                                                                                                                                                                               >  1:141600/1‑247 (MQ=255)                                                               cATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAACGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCg                                                                                                                                                           <  1:58412/251‑1 (MQ=255)                                                                                                                                                                                    cGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTACAAACCCCTAACAGAACGATGGCGCGACAGCGATTCCAGCATAAGGCCGAGATTGAACAGAACCATAATCATCGCGCTGGCTTCGTAATAAAGATGACGCGCTTCCATTGGGAACCACTGCGGCCACAGGTTGACCCTCATCGAATAACGACACGCCACGCCAGAACCACCCGCCCCCAGCGTaacaaacgacgccgccccg                                      >  1:90716/1‑233 (MQ=255)                                                                                                                                                                                                                        cGAGTAACTTTTCCAACGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATgg  >  1:89650/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            GGCATGGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGG  >  NC_000913/510210‑510675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑