Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,335,259 G→A D462D (GAC→GAT)  yfaA ← DUF2138 domain‑containing protein YfaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,335,2590GA88.9% 14.4 / ‑5.8 9D462D (GAC→GAT) yfaADUF2138 domain‑containing protein YfaA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (1/7);  total (1/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAGTTTCGCTATTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑GTCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCACTTCGCGACGCCAGATTTGCGCTTCGCCCTGCTGAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCT  >  NC_000913/2335022‑2335493
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
cATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAGTTTCGCTAGTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGGGTTATTAACCAGCGTAGT‑ATCGAGGgag                                                                                                                                                                                                                                <  1:142206/250‑1 (MQ=255)
                tgttgAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAGTTTCGCTATTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑ATCATCGAggg                                                                                                                                                                                                                                  <  1:45310/232‑1 (MQ=255)
                                                                         aCCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAGTTTCGCTATTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATGCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑ATCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTgcgn                                                                                                                                                      <  1:18094/251‑2 (MQ=255)
                                                                                                                     cagcagTTTCGCTATTCCTTGTGGATTGATATAGAGCGGACCGATGCCATTCGTGGGTATTACATCCCCCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTCTTAACCAGCGT‑ATCATCGAGGGAGAAAAGCCGCGCTTTGTTTTGCATCGCCCGCGAAACCCGGAAAAAAAAATACGAACTTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCCCTTcgc                                                                                                          >  1:120481/1‑251 (MQ=255)
                                                                                                                        cagTTTCACTATTCCTTGTTGATTGATATAGAGCGGAACGATGCCATCATTGGTTATTACCTCCCCCATTGCCGGCCGGGTTTGATTCAGTGTTTGCAGCGCGTTAGTAACCAGCTT‑ATCATCGATGGAGAAAACCAGCGTTTTGTTTTGCCTGGCCCGCGACACACGGAAAAAAGACTCCGACATGGAGTTAGCGGGTTGCGCCGCCTCTGCGTTCGGATACTGGCCGTATCGGGAACTCCCTTCGCGAc                                                                                                       <  1:87041/251‑1 (MQ=255)
                                                                                                                              cGCTATTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑ATCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCACTTCGCGACGCCAg                                                                                                  <  1:25409/250‑1 (MQ=255)
                                                                                                                                                                                 tACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑ATCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCACTTCGCGACGCCAGATTTGCGCTTCGCCCTGCTGAGTCTGGCTTACCGGCAAAACACCTTCTGgc                                               <  1:144178/250‑1 (MQ=255)
                                                                                                                                                                                 tACATCAACCATTGCCGGGCGGGTTTTAGTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑ATCATCGAGGGAGAAAAGCAGCGTTTTGTGTTGCAGCGACAGCGACACACGGAAAAAAGAATCCGACAGTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCTCTTCGCGACGCCAGAGTTGCGCTTCGCCCTGCTGAGTCTGGCTTACCGGCAAACCACCTTCTgga                                               <  1:98444/250‑2 (MQ=255)
                                                                                                                                                                                                                             cgTTATTAACCAGCGT‑ATCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTGCGCCGCCGGCGCTGTCGGAGACTGGCCGTATCGGGAACTCACGTCTCGACGCCAGATTTGCTCTTCGCCCGTCTCAGTCTGGCTTCCCGGCAAAACACCGTCTGGCGCTTCGCTTTCGTGCGCCCCAATATTTTGCGTAAACAGTTTCCCt  <  1:173108/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
CATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAGTTTCGCTATTCCTTGTGGATTGATATAGAGCGGAACGATGCCATCAGTGGGTATTACATCCACCATTGCCGGGCGGGTTTTATTCAGTGTTTGCAGCGCGTTATTAACCAGCGT‑GTCATCGAGGGAGAAAAGCAGCGTTTTGTTTTGCATCGCCAGCGACACACGGAAAAAATAATCCGACATTAATTGATCGGGTTGCGCCGCCTGCGCTTTCGGATACTGGCCGTATCGGGAACTCACTTCGCGACGCCAGATTTGCGCTTCGCCCTGCTGAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCT  >  NC_000913/2335022‑2335493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑