Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,681,779	G→A	R84R (CGC→CGT)	ydgC ←	GlpM family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,681,779	0	G	A	100.0%	17.7 / NA	8	R84R (CGC→CGT)	ydgC	GlpM family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/2);  total (6/2)

GGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAAC  >  NC_000913/1681600‑1681976                                                                                                                                                                                    |                                                                                                                                                                                                      ggCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATAATTCCGGTGAAATACCCCAGCGACACCAAGCAAACAAAATAGGGAATAATCGACCACATACTaa                                                                                                                                 >  1:162265/1‑250 (MQ=255)     ngaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATAACACAGCGACACCAGGTAAACAAAATAGGGAATAAACGACCACATACTAAAAat                                                                                                                             >  1:100556/2‑250 (MQ=255)                                                         cGGACGCAGTTTCACACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAGTCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgcg                                                                        >  1:132495/1‑251 (MQ=255)                                                          ggACGCTGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgcgc                                                                       <  1:150865/251‑1 (MQ=255)                                                                      ccACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACAACCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATACCACAGCGACACCAGGGAAACAAAATAGGGAAAAATCGCCCACCTACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCaa                                                            >  1:121456/1‑250 (MQ=255)                                                                                                      cAGCTTTATACAGAACATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGAAAACGCATCATTCCGGTGAAATACCACAACGACACCAGGAAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGAGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGatat                           >  1:128499/1‑251 (MQ=255)                                                                                                                                         ttATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAGTAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATAGAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCtt      <  1:166263/237‑2 (MQ=255)                                                                                                                                                    cacGCAACAGAACCAACAAAAGCGGCGGGCAAACGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAac  >  1:8143/1‑230 (MQ=255)                                                                                                                                                                                    |                                                                                                                                                                                                      GGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAAC  >  NC_000913/1681600‑1681976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑