Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,450,650	C→T	E234K (GAA→AAA)	tynA ←	copper‑containing amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,450,650	0	C	T	100.0%	19.7 / NA	8	E234K (GAA→AAA)	tynA	copper‑containing amine oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

TTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCATACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGG  >  NC_000913/1450442‑1450880                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       ttCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGTGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAtttttc                                                                                                                                                                                                                                     <  1:152035/212‑1 (MQ=255)              cAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCTGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTATGCACACTGGCGAAATCATCCAGCAACACCAt                                                                                                                                                                                  >  1:121615/1‑250 (MQ=255)                                                 cAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGAATCTTGTTTCAGGCCATATTTAACATCGAACTAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTAACGGCAGCGGCAAATTTTTCACTGATGTTAATAATGTTCTGCACACTGGCGAAATCATCAAGCAACACCATACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGc                                                                                                                                                >  1:26794/1‑249 (MQ=255)                                                            aTCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTAGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCg                                                                                                                                                                                                      >  1:155362/1‑184 (MQ=255)                                                                                                                         ttACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCATACCGTGGTCGTCTTTAATGTGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTg                                                                        <  1:25834/249‑1 (MQ=255)                                                                                                                                                              tCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCTAAATCATCCAGCAACACCATACCGTGGGCGTCTTTAATGTGTTGCCAGGAGAGCAGTTGGTTGTTTTGCAGATCCACCGCCGCGTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCt                                                                 <  1:148690/219‑1 (MQ=255)                                                                                                                                                                                 aaTACCGCGTTTCTTCACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCATACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTg              >  1:84950/1‑251 (MQ=255)                                                                                                                                                                                                cACGGCAGCGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCCTACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACAACCGCTTCGATGATATGTTTGCCGTCGCGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAggg  >  1:149780/1‑248 (MQ=255)                                                                                                                                                                                                       ncGGCAAATTTTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCATACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGatat                                                                                                        >  1:42539/2‑139 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       TTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACACCATACCGTGGGCGTCTTTAATGGGTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGG  >  NC_000913/1450442‑1450880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑