Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,205,728	C→T	H12Y (CAT→TAT)	yehM →	uncharacterized protein YehM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,205,728	0	C	T	100.0%	25.1 / NA	10	H12Y (CAT→TAT)	yehM	uncharacterized protein YehM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

GGGCGTCCGCGCCTGGTTTTTAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGCAGCCCCCGGGCGTGGTGCCTGGACGCCATTTGCTG  >  NC_000913/2205492‑2205929                                                                                                                                                                                                                                             |                                                                                                                                                                                                          gcagacgtgtgctctcccgattgCGAACCGCGCGGGGGAGCTTGCCGCCCTGGGGGAAGGTATTGCCGGAACCAGCGACAAAGATAACGAAGAAGATCGCGCGCGTCTGCCCCGTTACTTGGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGTTAATTATCAAGCCCGCCCCCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTgc                                                                                                                                                                                             <  1:2702/229‑1 (MQ=255)                              gcgcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGaa                                                                                                                                                                  >  1:45995/1‑249 (MQ=255)                                gcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCTTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACAGGCAGCCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGAAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGGCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGcc                                                                                                                                                              <  1:107472/251‑1 (MQ=255)                                     ggAGCCTGCCGACCTGGTGGAAGTTATTGCCGGAACCTTCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTTTTATCAAGCCCGCTACCGCCCGCCGGGAGGAAATATGCAGGAGCGAGGCGTTAATTGTCTGCATGTGGCATTATAGTCCGGTCTGCGGCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCgg                                                                                                                                                         <  1:81395/251‑1 (MQ=255)                                                                                                                      nttCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGACGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGACTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGAGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTACTGACCGGGTAGACGAACTGTTTTTAGCACACCAGCTTCCGGt                                                                         >  1:31268/2‑250 (MQ=255)                                                                                                                                                                              cccGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGGGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGCAGCCCCCGGGCGTGGTGCCTg                <  1:88481/251‑1 (MQ=255)                                                                                                                                                                                  ccACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGCAGCCCCCGGGCGTGGTGCCTGGACg            >  1:45582/1‑251 (MQ=255)                                                                                                                                                                                             cGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCACGGCTGGTGAAATCGTTAATCGACAGCCAGCGGCCACGATACGTGTTGATTGACGGCCCGGCTGATTTTAATGACCGGGTAGTCGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGCAGCCCCCGGGCGTGGTGCCTGGACGCCATTTGCTg  >  1:167165/1‑250 (MQ=255)                                                                                                                                                                                                          ncATGAGCGAGCCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGc                                     >  1:105574/2‑202 (MQ=255)                                                                                                                                                                                                                gcgagcCGTTAATTGTCGGCATCCGGCATTATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATANNTGttgan                                                                                                                                        >  2:12817/1‑96 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                          GGGCGTCCGCGCCTGGTTTTTAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTTAGCCCACCAGCTTCCGGTAGCTATTTACAGTTATTGCCAGTATCAGGACGGTGCAGCCCCCGGGCGTGGTGCCTGGACGCCATTTGCTG  >  NC_000913/2205492‑2205929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑