Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,897	C→T	A109T (GCG→ACG)	recF ←	recombination mediator protein RecF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,897	0	C	T	100.0%	27.0 / NA	13	A109T (GCG→ACG)	recF	recombination mediator protein RecF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/9);  total (4/9)

CGCCAGCGGGATCAGCTCTTTATCCCACGGGCGTAGCTGTTCGTAACGTGTCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCAC  >  NC_000913/3880667‑3881121                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 cGCCAGCGGGATCAGCTCGTTATCCCACGGGCGTAGCGGTTCGTAACGTGACACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCGCCAGGCGGTGAAAAACCCGGGTTCTGTGGGAAAGGATCCCCTGTCGAGGAATACTCATCTGGAGTTGGGGCTGCCGGTGAGGAAAGTAAACGCGTCTGGCGTGATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCGTATg                                                                                                                                                                                                                <  1:125794/249‑1 (MQ=255)                      aTCCCACGGGCGTAGCTGTTCGTAACGTGTCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGATTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGTGACGCCGTTGAGTAAAGTAAACCCTTCGGGCGTTATCACCTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGa                                                                                                                                                                                         <  1:76400/251‑1 (MQ=255)                                                                                                          ggCGGTGAAAAATCCGGGTTTGTTGTGAAAGCATCCCCAGTAGAGGAATGCTCTTCTGTATTTTGGGCCGCCGTTGAGTTAAGTAAACCCTTCTGGCGTTATCCACTGCATTGGCCTCAGGTGCGTAAGTTCCGCGACCTTATGCCCCTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACg                                                                                                     >  1:126181/1‑251 (MQ=255)                                                                                                              gTGAAAAATCCGGGTTCGTTGTGCAAGCATCCCCAGTAGAGGAATGCTCTTCTGTCTTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCACCTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCt                                                                                                                             >  1:50892/1‑223 (MQ=255)                                                                                                                gAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGt                                                                                                                                                             <  1:70768/189‑1 (MQ=255)                                                                                                                            ttCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGc                                                                                                                                                                                   <  1:139844/155‑1 (MQ=255)                                                                                                                                             cccAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCAGGGCGAATGACGCGACCAATCTGc                                                                  <  1:47053/251‑1 (MQ=255)                                                                                                                                                    gAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTATGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCAGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGATGTCTCGCGCTCTTCGCCCTGTACTCGCCCGTGgagta                                                                                                           >  1:155684/1‑201 (MQ=255)                                                                                                                                                                 ctGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGAc                                                                               >  1:55645/1‑218 (MQ=255)                                                                                                                                                                   gTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTg                                                                                                                                                                                 <  1:112784/118‑1 (MQ=255)                                                                                                                                                                                            gtaaaCCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCGCCTGCTCATGGCGAATGACGCGACCAATCTGTAAACTGCGAAACGCCCGACCATGGc                                         <  1:137041/229‑1 (MQ=255)                                                                                                                                                                                                     tCTGGCGTTATCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTgg           <  1:78661/250‑1 (MQ=255)                                                                                                                                                                                                              aTCAACTGCATTGGCATCAGGTGCGTCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCan  <  1:19929/250‑2 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 CGCCAGCGGGATCAGCTCTTTATCCCACGGGCGTAGCTGTTCGTAACGTGTCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCAC  >  NC_000913/3880667‑3881121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑