Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,182	G→A	G169E (GGG→GAG)	rep →	ATP‑dependent DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,182	0	G	A	100.0%	13.3 / NA	6	G169E (GGG→GAG)	rep	ATP‑dependent DNA helicase Rep
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/5);  total (1/5)

ATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGAGCGGGACCGTATTTTTGCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG  >  NC_000913/3961005‑3961313                                                                                                                                                                                  |                                                                                                                                    aTACCGAGCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACGGATTTCGACCATCTCGAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGAGCGGGACCGTATTTTTGCCCATTGTGATGAGCTGTATGATGCACACCGGAATGGCTGTAACGTGCTCGACTTCGATGATCTGATTTTATTGCCGACGTtgctg                                                            <  1:62536/251‑1 (MQ=255)   aCCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGAGCGGGACCGTATTTTTGCCCATTGTTATGAGCGGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTtgctgct                                                          <  1:129995/251‑1 (MQ=255)          aGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCAGCTCTAACTGGAAGAATGATCTCATAACACCGTTCCTGTCGGCAGCAAGTGCGATTGGCGAGCGGGACCGGATTTTTGCCCAGTGTTATGAGCTGTATTATGCACACCTTAAAGCCTTTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGc                                                   <  1:28325/251‑1 (MQ=255)                                                           aGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGAGCGGGACCGTATTTTTGCCCATTGTTATGAGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGAGCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCGATCTGCtgg  <  1:123263/251‑1 (MQ=255)                                                                                                                                                   gAGCGGGACCGTATTTTTGCCCATTGTTATGAGCTGTATGCTGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                 >  1:114853/1‑148 (MQ=255)                                                                                                                                                   gAGCGGGACCGTATTTTTGCCCATTGTTATGAGCTGTATGATGCACACCTGAAAGCCGGNAACNTTCTNGANTTCGATGATCTGATTNTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGNNCNNNCNGAACaaaa                 <  2:114853/148‑1 (MQ=255)                                                                                                                                                                                  |                                                                                                                                    ATACCGATCAGCTTGCTTTGCTTAAAGAGTTGACCGAGGGGCTGATTGAAGATGACAAAGTTCTCCTGCAACAACTGATTTCGACCATCTCTAACTGGAAGAATGATCTCAAAACACCGTCCCAGGCGGCAGCAAGTGCGATTGGCGAGCGGGACCGTATTTTTGCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG  >  NC_000913/3961005‑3961313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑