Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,887	Δ1 bp	coding (678/1221 nt)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,887	0	C	.	100.0%	36.4 / NA	10	coding (678/1221 nt)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (5/5);  total (5/5)

AACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGG  >  NC_000913/1667696‑1668110                                                                                                                                                                                                |                                                                                                                                                                                                                                aaCATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTtgt                                                                                                                                                                                                                    >  1:11931/1‑204 (MQ=255)            cATGGATTGCTTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAg                                                                                                                                                                           >  1:47449/1‑234 (MQ=255)                              cAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCt                                                                                                                                        <  1:75880/251‑1 (MQ=255)                                            cAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACTGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCGTCGTGTAGCAGGTGACCATCGGTAATGACGCC‑GCGCCCACGTTGTGATCGATAACCACCTGAATCACATcgc                                                                                                                                                                                         <  1:88342/188‑1 (MQ=255)                                                                             ggCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGTCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAAc                                                                                                             >  1:142332/1‑231 (MQ=255)                                                                             ggCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAgcn                                                                                         <  1:19931/251‑2 (MQ=255)                                                                               cAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCCCGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCCCCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGcc                                                                                       <  1:12981/251‑1 (MQ=255)                                                                                                          tnNCCATACNNGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTtgt                                                                                                                                                                                                                    <  2:11931/99‑1 (MQ=255)                                                                                                                                                             cctgcGTGTAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGCATCACATCGCGCGACCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCa                                                                                            >  1:75103/1‑168 (MQ=255)                                                                                                                                                                    gtAGCAGATGACCATCGGTAATGACGCC‑GCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCactg  >  1:9873/1‑248 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                AACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAGCGCCTCGCCGAGCGGCATCTCTTTTACATCCTCGTAGAACGGCATGCGATGTACAATACCATTTTCCGTATCAATAATTCCCGGCAAGG  >  NC_000913/1667696‑1668110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑