Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,052,851	C→T	I269I (ATC→ATT)	hemN →	coproporphyrinogen III dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,052,851	0	C	T	100.0%	25.6 / NA	10	I269I (ATC→ATT)	hemN	coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CCATGCGCGTGAGATTGGTTTTACCTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGT  >  NC_000913/4052632‑4053080                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      ncATGCGCGTGAGATTGGTTTTACCTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAACATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAACCCATCGCCTTCCTGACGCAAt                                                                                                                                                                                                        >  1:99425/2‑251 (MQ=255)                        ccTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCAGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCCGTTTATCGGTATg                                                                                                                                                                                 >  1:61499/1‑251 (MQ=255)                                               tACGGCCTGCCGAAACAGACGCCGGANAGTTTCNNCTNTACCCTGAAACGTGTGGCGNAGNNNANNNCNCATCGNCTNNGTGTCTTTANCTACGCGCATCGTCCGGCCATTTTTGCTGCGCAGCGCAAAAGCGAAGGTTCAGNCCTGCCTAANNCGCGGCAAAAGCTCGATGTTCNGCANNGNNCNATCNcc                                                                                                                                                                                                                     >  2:39459/1‑192 (MQ=255)                                                                                 cctgtACCCGGAAACGTGTGGCGGAGCTGAACCCCGATCGGCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCTCTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGtgctn                                                                                                                        <  1:100144/247‑2 (MQ=255)                                                                                            nnaCNNNNNGCGNGGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACACGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGc                                                                                                                                                                                                                      <  2:101805/144‑1 (MQ=255)                                                                                                                          ntGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGCCCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAAc                                                                                                               >  1:32568/2‑219 (MQ=255)                                                                                                                                                                 gctgctCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGATGGGGATGGGCGTTTCCGCCATCAGCa                                               >  1:550/1‑244 (MQ=255)                                                                                                                                                                             aaaaTCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgc                            <  1:39459/251‑1 (MQ=255)                                                                                                                                                                                                       tCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGt  >  1:55476/1‑251 (MQ=255)                                                                                                                                                                                                         cGCAGCAAACACTCGATATTCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATAAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGc                                                                                                                                                 >  1:71113/1‑106 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      CCATGCGCGTGAGATTGGTTTTACCTCCACCAACATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGT  >  NC_000913/4052632‑4053080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑