Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,308	G→A	intergenic (+161/+50)	narP → / ← ccmH	DNA‑binding transcriptional dual regulator NarP/holocytochrome c synthase CcmH component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,308	0	G	A	100.0%	13.2 / NA	7	intergenic (+161/+50)	narP/ccmH	DNA‑binding transcriptional dual regulator NarP/holocytochrome c synthase CcmH component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

CGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGATTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCG  >  NC_000913/2291079‑2291481                                                                                                                                                                                                                                      |                                                                                                                                                                              cGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATACAAATAGCCCGATGGATTTATCATCGGGCTGAGATTTATGCCAAACGCAAAAATGCCTGATGCGCTCCGCTTATCAGGCCTACGTGGATCGATCACTTTATTGAATTTAAACAATTTTGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCATACACTAAACGCACTTTGTCAACAGTCt                                                                                                                                                            >  1:20811/1‑249 (MQ=38)  gTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACCATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGAATTACGACAAACGCATAACTGCCTGATGCGCTACGCTTATCACGCCTTCGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAACGCACTTTGTCAACAGTCTAGc                                                                                                                                                         >  1:166677/1‑251 (MQ=38)          aaTGTCCGCGCCCGCGGAGCGGCCACCATTCTGTTCCTGCAACAACGCGGTACACAATAAAAATAGCCCGATGGATTTAGCATCGGGCTGAGATTTATGACAAACACAAAACTGCCGGATGCGCTACGCTTATCAGGCTTACGTGGATCGATCAATTTATTGAATGTACACAAGTGTGTAGGCCGGATAAGGCATTCACGCCTCATCCGGCATAAACTAAACGCACTGTGGCAACAGTCTAGCCCGATGGc                                                                                                                                                 <  1:108123/251‑1 (MQ=255)                                                                aaaTAAAAATAGCCCGATGGATTTATCATCGGGCTAAGTGTTATGACAAACGCAAAACGGCCTGATGCGCTACCCTTATCAGGCCTAGGTGGATCGATCAATGTAGTGAATGTACACAATTTTGTAGGCCGGATGAGTGGTGCGCGCCGCAGCCGGCATAAACTAAACGAACTTTGTCGACAGGCTAGCCCTAGAGCATCACCATCGGGCCGCTTTTTGTTTACTCTCCTGCGGCGatctggtcgatgggc                                                                                           <  1:108524/250‑15 (MQ=255)                                                                            cccGATGGATTTATCATCGGGCTGAGATTTATGACAAACGAAAAACTGCATGATGCGCTACGCTTATCAGGGCTACGTGGATCGCTAAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAACGCACTTTGTCAACAGTCTAGCCCGATGGCATCAACATAGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGCtac                                                                               >  1:111033/1‑251 (MQ=38)                                                                                                                                                   ccTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCATAACCTAAACGCGCTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCCCCGCACGGCGAGTATCGTTGGCCGGTAAGAGATTCAACATCATCTCCCACGCGGCAACg        >  1:67210/1‑251 (MQ=255)                                                                                                                                                                                                                  ccgcATCCGGCATAAACTAAACGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACACATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCg  >  1:74642/1‑194 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                              CGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATAAAAATAGCCCGATGGATTTATCATCGGGCTGAGATTTATGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCG  >  NC_000913/2291079‑2291481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑