Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	505,321	G→A	P136P (CCG→CCA)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	505,321	0	G	A	100.0%	11.7 / NA	6	P136P (CCG→CCA)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

CGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTTAAACCGTGGGCGCTGTTTAAGCGTCAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCG  >  NC_000913/505086‑505501                                                                                                                                                                                                                                            |                                                                                                                                                                                     cgcAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGCCTGAATTACAGGCTGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCATTGCTTTCCGCGAat                                                                                                                                                                       >  1:78985/1‑251 (MQ=255)                            gCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCATTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAgcgc                                                                                                                                            <  1:137600/251‑1 (MQ=255)                                                                                                                      ccGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCATTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTTAAACCGTGGGCGCTGTTTAAGCGTCAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAAttn                                                       <  1:135327/246‑2 (MQ=255)                                                                                                                                      cgGTATGAATCTGGGGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCATTGCTGTCCGCGAATATCTCCCAGAAAAGTACTGGCGAGCGCCTGGTTAAACCGTGGGCGCTGTTTAAGCGTCAGGATCGGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTg                                  <  1:116903/251‑1 (MQ=255)                                                                                                                                            gAATCTGGTGTGCTATGACGCGATGGCGATCGGTAAGCATGAATGTGATAATCCGCTCACCGTATTACGCCAGCAGTAAAAGTGGGCCAAGTTCCCATTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGGGAGCGCCGGTTTAAGCCGTGGGCTCTGATTAAGCGTCAGTATCTTAAAATTGTCTGTATTGGGCTGACAACCGATGACTCAGCAAAAATTTTTATCCCGGAATACTTCACTGATATCg                            <  1:62378/251‑1 (MQ=255)                                                                                                                                                                       gATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCCAGTTCACATTGCTTTCCGCGAATATCTACCAGAAACGTACTGGCGAGCGCCTGTTTACACCGTGGGCGCTGTTTAAGCGTCAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAACACTTGGTAACCCGGAATACTTCACTGATATCGAATTTAGTAAGCCCGCCGATgaagcg  >  1:68637/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                     CGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTTAAACCGTGGGCGCTGTTTAAGCGTCAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCCGGAATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCG  >  NC_000913/505086‑505501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑